氟苯尼考制剂中磺胺二甲嘧啶和磺胺间甲氧嘧啶检测方法的研究

Study on Detection Method of SM2 and SMM in Florfenicol Preparations

为建立测定氟苯尼考制剂中非法添加的磺胺二甲嘧啶（SM2）和磺胺间甲氧嘧啶（SMM）高效液相色谱分析方法,本研究使用C18色谱柱,以乙腈-0.2%冰醋酸溶液（27∶73）作为流动相进行等度洗脱（流速1.0 mL/min）;采用二极管阵列检测器,检测波长270 nm;进样量20μL。结果表明,磺胺二甲嘧啶（SM2）和磺胺间甲氧嘧啶（SMM）分别在进样浓度为31.25~500.00μg/mL和12.50~200.00μg/mL范围内呈良好线性关系（r2分别为0.9996和1.0000）;平均回收率分别为98.6%（SM2）和99.8%（SMM）;检出限分别为0.125μg/mL（SM2）和0.225μg/mL（SMM）。建立的方法简便、准确、可行,适用于氟苯尼考制剂中非法添加的磺胺二甲嘧啶和磺胺间甲氧嘧啶的检测。

A detection method of SM2 and SMM illegally adulterated in florfenicol preparations were established by high performance liquid chromatography（HPLC）.The chromatography was performed with C18column at 270 nm using PDAD.The mobile phase consisted of a mixture of acetonitrile-0.2% acetic acid（27∶73） at a constant flow rate of 1.0 mL/min.The injection volume was 20 μL.The results showed that there were good linear relations（with r2as 0.9996 and 1.0000 respectively） when the injection concentration was 31.25 ~ 500.00 μg/mL（SM2） and 12.50 ~ 200.00 μg/mL（SMM） repectively.The average recovery rate was 98.6%（SM2） and 99.8%（SMM）.The detection limit was 0.125 μg/mL（SM2） and 0.225μg/mL（SMM） respectively.This method was simple,accurate and feasible and was suitable for detection of SM2 and SMM illegally adulterated in florfenicol preparations.