DNA氧化损伤对人外周血淋巴细胞微核率的影响

Effect of DNA oxidative damage on micronucleus frequency in peripheral blood lymphocytes

目的探讨H2O2诱导淋巴细胞氧化损伤对其微核率的影响。方法采用10、50、100、1 000μmol/L H2O2诱导静息期淋巴细胞DNA损伤;体外培养淋巴细胞,有丝分裂原刺激其进入细胞周期,于培养不同时间点加入1 000μmol/L H2O2诱导DNA损伤,常规培养法检测微核率的变化。结果各实验组微核率并无明显变化。结论 H2O2可以诱导淋巴细胞DNA损伤,但对微核的形成无影响,可能与DNA损伤类型及快速修复有关。

Objective To study the effect of DNA damage induced by H2 O2 on the micronucleus frequency in lymphocytes. Methods Resting lymphocytes were treated with different levels of H2 O2 （10,50,100,1 000 μmol/L）.1 000 μmol/L H2 O2 was added into mitogen-stimulated lymphocyte cultures at different time intervals.Then micronucleus rate was examined by the conven-tional culture method.Results There was no significant change of the micronucleus frequency in the experimental groups.Conclu-sion H2 O2 could induce lymphocyte DNA damage rapidly,but exerts no effect on the formation of micronuclei,which may be relat-ed to the type of DNA damage and rapid DNA repair.