DNA微阵列芯片法用于检测结核分枝杆菌耐药性的研究

Research on DNA microarray chip method for detecting drug resistance of Mycobacterium tuberculosis

目的通过与传统的比例法在结核分枝杆菌耐药性检测的比较,评价DNA微阵列法用于检测结核分枝杆菌耐药性的可行性。方法随机抽取本院2012年1月至2013年3月从临床标本中分离培养所得的结核分枝杆菌54株,通过DNA微阵列法和比例法分别进行异烟肼和利福平的耐药性检测并对结果进行比较分析。结果以比例法为金标准,DNA微阵列法对异烟肼和利福平的耐药检测结果与比例法的符合率分别为75.0%、91.0%。结论 DNA微阵列技术适用于临床一线耐药结核分枝杆菌的快速筛查。

Objective To evaluate the feasibility of the DNA microarray method used in detecting the drug resistance of Myco-bacterium tuberculosis by comparing the traditional proportion method and the DNA microarray method for detecting the drug re-sistance of Mycobacterium tuberculosis.Methods 54 strains of Mycobacterium tuberculosis isolated from clinical specimens in our hospital from January 2012 to March 2013 were randomly extracted and their resistance to isonicotinic acid hydrazide （INH）and rifampicin（RFP）was detected by the DNA microarray method and the proportion method.The detection results were performed the comparative analysis.Results With the proportion method as the golden standard,the coincidence rates of the DNA microarray method for detecting the Mycobacterium tuberculosis resistance to INH and RFP were 75% and 91.0% respectively.Conclusion The DNA microarray technique is suitable for the rapid screening of clinical first-line drug resistant Mycobacterium tuberculosis.