毛细管电泳法分析糖基化血红蛋白

Analysis of glycosylation hemoglobin by capillary electrophoresis

目的探讨毛细管电泳在分析糖基化血红蛋白水平方面的临床应用价值。方法该文利用长30cm、内径25μm的未涂层熔融石英毛细管,利用血红蛋白在415nm波长处的特异性吸收峰,二极管阵列(PDA)法检测健康人与糖尿病及高危人群中的糖基化血红蛋白(HbA1c)水平。在研究过程中,对精密度和准确度进行了精确分析。结果 HbAlc和非HbAlc成功分离,峰型清晰锐利且能对HbAlc进行定量分析。结论毛细管电泳法具有特异性好、精密度和准确度高等优点,因此,该法应该在临床上被推广应用。

Objective To discuss the application value of the capillary electrophoresis in detecting the glycosylation hemoglobin （HbA1c）level.Methods By utilizing the uncoated fused silica capillary column（30 cm in length and 25 μm in diameter）and the specific adsorption peak,the HbA1c levels in the healthy people,patients with diabetes mellitus（DM）and high risk people were de-tected by the photodiode array（PDA）method at the wavelength of 415 nm.During the research process,the precision and accuracy were perfomred the accuracy analysis.Results HbA1c could be effectively separated from non-HbA1c,with a sharp peak.And the relative quantity of HbA1c could be determined.Conclusion The capillary electrophoresis is characterized by specificity,high precision,and high accuracy.Therefore,it should be widely applied in clinic.