实时荧光定量PCR与头孢西丁筛选试验在耐甲氧西林金黄色葡萄球菌检测中的评价

Evaluation of real-time fluorescent quantitative PCR and cefoxitin screening test in detection of methicillin-resistant Staphylococcus aureus

目的以实时荧光定量PCR(RT-PCR)技术检测耐甲氧西林葡萄球菌(MRSA)mecA基因为金标准,进行与头孢西丁纸片筛选试验的评价。方法收集各种细菌感染性标本分离到的葡萄球菌,分别采用PCR扩增mecA法、头孢西丁筛选试验检测MRSA。结果 RT-PCR检出金黄色葡萄球菌中携带mecA基因占54.55%(66/121)。与VITEK2细菌鉴定仪头孢西丁筛选试验的结果比较差异无统计学意义(P>0.05)。头孢西丁筛选试验敏感度93.93%,特异度81.82%,阳性预期值84.93%,阴性预期值91.83%。结论 RT-PCR技术可准确、快速检测MRSA。

Objective To evaluate the cefoxitin screening test in detecting（MRSA）with the real-time fluorescent quantitative polymerase chain reaction（RT-PCR）for detecting mecA gene as the golden standard.Methods Staphylococcus aureus strains iso-lated from various bacterial infection specimens were collected,which were amplified by RT-PCR and detected by the cefoxitin screening test respectively.The results were compared.Results In 121strains of MRSA,66 strains carrying mecA gene were detec-ted by RT-PCR（P 〉0.05）,accounting for 54.55%（66/121）.There was no statsitcal difference in the detection results between the cefoxitin screening test and RT-PCR.The sensitivity of the cefoxitin screening test was 93.93%,the specificity was 81.82%,the positive predictive value was 84.93% and negative predictive value was 91.83%.Conclusion The RT-PCR technique can accurate-ly and rapidly detect MRSA.