鼠尾胶原在毛细胞膜片钳实验中的应用

Application of rat tail collagen in patch clamp experiment with vestibular hair cells

目的 探讨应用鼠尾胶原在豚鼠前庭毛细胞膜片钳实验中的促进毛细胞贴壁效果。方法 制作鼠尾胶原 ,观察全细胞膜片钳实验中 ,自制的鼠尾胶原对前庭毛细胞的贴壁黏附作用。结果 无鼠尾胶原时 ,前庭毛细胞悬浮于外液 ,不宜封接 ;有鼠尾胶原时 ,前庭毛细胞贴附于培养皿底壁 ,易于封接和长时间 (约 8h)的观察和记录。鼠尾胶原对前庭毛细胞具有良好的贴壁黏附促进作用。结论 鼠尾胶原能促进毛细胞贴壁 ,涂布鼠尾胶原有利于膜片钳实验的长时程记录 ;并且制作简便 。

Objective To study the feasibility of application of rat tail collagen in patch clamp research in vestibular hair cells.Methods The effect of self made rat tail collagen on promoting adhesion of vestibular hair cells in whole cell patch clamp experiment was observed.Results A seal was hard to be formed when the vestibular hair cells suspended among the external solution without rat tail collagen,However,when the vestibular hair cells were firmly adhesive to the bottom of the recording chamber with rat tail collagen,a seal can be formed easily,which fitted to the long term observation and recording.The effect of rat tail collagen on adhesive to vestibular hair cells is obvious.Conclusion Rat tail collagen could facilitate the vestibular hair cells to adhesive with the bottom of the recording chamber,which is helpful for long term patch clamp research,and the collagen is considered as an optimal adhesive reagent to vestibular hair cells for patch clamp research.