LC-MS/MS测定人参蜂王浆口服液中15种药物残留

Determination of 15 Drug Residues in Ginseng and Royal Jelly Oral Liquid by HPLC-MS/MS

建立了同时测定人参蜂王浆口服液中15种抗生素药物(6种喹诺酮类、4种四环素类、3种大环内酯类、2种林可胺类)残留的高效液相色谱-串联质谱(LC-MS/MS)检测方法。该方法采用pH8.0磷酸盐缓冲液提取样品,提取液经HLB固相萃取柱净化。以甲醇-乙腈-0.1%甲酸作为流动相,C18色谱柱对15种药物进行分离,电喷雾正离子多反应监测模式进行检测。方法检测限为0.02ng/mL^0.9ng/mL,线性范围0.08ng/mL^20ng/mL,加标回收率为71.8%~87.8%,相对标准偏差为2.2%~7.9%(n=9)。该方法准确、灵敏、简便,适用于人参蜂王浆口服液中四环素等药物残留的测定。

A HPLC-MS/MS method was established for simultaneous determination of 15 drug residues （6 quinolones,4 tetracyclines,3 macrolides,lincomycin and clindamycin）in Ginseng and Royal Jelly Oral Liquid. Samples were extracted with phosphate buffer solution （pH=8.0）and then purified by Oasis HLB solid phase extraction column. Chromatographic separation was carried out on a C18 column,the mobile phase is methanol-acetonitrile-0.1%formic acid water solution with gradient elution, then detected by MS/MS in the ESI（＋）-MRM mode.The limits of detection for the method were 0.02 ng/mL-0.9 ng/mL,The linear plots were obtained between 0.08 ng/mL-20 ng/mL,recoveries ranged from 71.8%-87.8%,and relative standard deviations were in the range of 2.2%-7.9%（n=9）.