霍山石斛安全性评价研究

Study on Toxicological Security of Dendrobium huoshanense

对霍山石斛进行食用安全性评价。采用气相色谱法测定有机氯农药残留量;原子吸收分光光度法测定重金属含量;通过动物急性毒性试验、遗传毒性试验(Ames试验、小鼠骨髓微核试验、小鼠精子畸形试验)和30d喂养试验进行毒理学评价。霍山石斛的有机氯农药残留量和重金属含量均符合国家相关标准;雌雄小鼠经口MTD均大于30g/kg·bw,Ames试验、小鼠骨髓微核试验、小鼠精子畸形试验三项遗传毒性试验结果均为阴性。30 d喂养试验未见大鼠的生长发育、血液学、生化、脏体比及组织病理学有异常变化。霍山石斛无急性毒性、亚急性毒性与致突变作用;在本实验剂量范围内,霍山石斛属安全性保健食品原料。

To provide the toxicological evaluation for safety of Dendrobium huoshanense. The contents of organic chlorine pesticide residue and heavy metals were determined by gas chromatography and atomic absorption spectrophotometer;Acute toxicity test, genetic toxicity test （Ames test, micronucleus test of born marrow in mice, sperm shape abnormality test in mice） and thirty-day feeding test in rats w ere conducted in this study. The contents organic chlorine pesticide residue and heavy metals all measure up to national standard. The oral MTD in both male and female mice were more than 30.0 g/kg＆#183;bw, the results of Ames test , micronucleus test of born marrow in mice, sperm shape abnormality in mice were negative . The thirty-day feeding test in rats demonstrated that it had no obvious toxic effects on growth and development, routine blood , biochemical index , VSI and histopathological changes. Dendrobium huoshanense has no significant acute and Sub-aeute toxicity and mutagenic effect and is safe as a health food.