摘要
以分光光度法测定非洲马铃果种子总生物碱、总黄酮、水溶性多糖含量,MTT法测定乙醇提取物不同分离部位的细胞毒活性,以及DPPH法测定不同分离部位的抗氧化活性。结果表明,非洲马铃果种子总生物碱、总黄酮、水溶性多糖含量分别为5.70%、0.41%和3.04%;各分离部位中总生物碱的细胞毒活性最强,其对人的肝癌细胞株SMMC7721、胃癌细胞株SGC7901和正常肝细胞株Chang-liver的IC50值分别为11.99±2.22、8.72±6.11和7.31±4.39μg·mL-1;各分离部位中正丁醇相抗氧化活性最强,IC50值为0.268±0.002 mg·mL-1。
In this study, the total alkaloids, total flavonoids and water-soluble polysaccharides of seeds of Voacanga africana native to Ghana were determined by means of spectrophotometry, cytotoxicity and antioxidant activity of different isolation from ethanol extract by MTT and DPPH method, respectively. The results revealed that the content of total alkaloids, total flavonoids and water-soluble polysaccharides were 3.04%, 0.41% and 3.04%, respectively. The alkaloid isolation exhibited strong cytotoxity against the human hepatocarcinoma cells SMMC7721, the human gastric carcinoma cells SGC7901 and human normal liver cells Chang-liver and the IC 50 values were 11.99±2.22, 8.72±6.11 and 7.31±4.39 μg·mL-1 respectively. The n-butanol isolation displayed antioxidant activity and the IC 50 value was 0.268±0.002 mg·mL-1.
出处
《亚热带植物科学》
2014年第2期98-102,共5页
Subtropical Plant Science
基金
厦门市科技创新资金项目(3502Z20101016
3502Z 20122011)