有柄石韦总多酚含量测定及其超声提取工艺优化

Polyphenols Extraction from Pyrrosia petiolosa by Ultrasonic and Its Content Determination

为优选有柄石韦Pyrrosia petiolos中总多酚超声提取工艺，采用Folin-Ciocalteus比色法对总多酚进行含量测定，通过单因素和正交试验，对提取工艺参数进行优化。结果表明，没食子酸对照品在浓度为1～10 mg·L-1范围内，与吸光度呈良好线性关系（y=2.406x-0.012，R=0.9994），有柄石韦总多酚的最佳提取工艺条件为：55%乙醇，料液比1∶10（W/V），超声提取60 min，超声提取温度75℃，总多酚提取率为21.47 mg·g-1。

To optimize the extraction of total polyphenols in Pyrrosia petiolosa by ultrasonic, the content of the polyphenols was used as investigation index, and the total polyphenol content was determined by Folin-Ciocalteus colorimetric. The extraction process parameters for polyphenols were optimized by the single factor and the orthogonal experiment. The results showed that there was a good linearity between gallic acid concentration and absorbance in the range 1~10 mg·L-1 （y=2.406x-0.012, R= 0.9994）;The optimum conditions were as follows： solvent concentration 55%, ultrasound time 60 min, extraction temperature 75℃, solid to liquid ratio 1∶10. The polyphenols extraction rate was 21.47 mg·g-1 in the best technical conditions.