摘要
为优选有柄石韦Pyrrosia petiolos中总多酚超声提取工艺,采用Folin-Ciocalteus比色法对总多酚进行含量测定,通过单因素和正交试验,对提取工艺参数进行优化。结果表明,没食子酸对照品在浓度为1~10 mg·L-1范围内,与吸光度呈良好线性关系(y=2.406x-0.012,R=0.9994),有柄石韦总多酚的最佳提取工艺条件为:55%乙醇,料液比1∶10(W/V),超声提取60 min,超声提取温度75℃,总多酚提取率为21.47 mg·g-1。
To optimize the extraction of total polyphenols in Pyrrosia petiolosa by ultrasonic, the content of the polyphenols was used as investigation index, and the total polyphenol content was determined by Folin-Ciocalteus colorimetric. The extraction process parameters for polyphenols were optimized by the single factor and the orthogonal experiment. The results showed that there was a good linearity between gallic acid concentration and absorbance in the range 1~10 mg·L-1 (y=2.406x-0.012, R= 0.9994);The optimum conditions were as follows: solvent concentration 55%, ultrasound time 60 min, extraction temperature 75℃, solid to liquid ratio 1∶10. The polyphenols extraction rate was 21.47 mg·g-1 in the best technical conditions.
出处
《亚热带植物科学》
2014年第2期108-110,共3页
Subtropical Plant Science
基金
山东中医药大学2013年SRT
关键词
超声法
有柄石韦
总多酚
提取工艺
正交试验
ultrasonic
Pyrrosia petiolosa
polyphenols
extracting technique
orthogonal experiment