输卵管炎种鸭大肠杆菌的分离鉴定及生物学特性分析

Identification and Characterization of Escherichia coli Isolates from Salpingitis Duck

为分析表现输卵管炎种鸭体内分离的大肠杆菌的致病性、药敏试验、血清型及毒力基因携带情况,无菌采集表现输卵管炎的种鸭肝脏,进行大肠杆菌的分离鉴定,经易感鸭皮下注射,测定其致病性;纸片法进行药敏试验;凝集试验确定血清型;PCR法检测毒力基因。结果共分离鉴定大肠杆菌16株,其中强毒菌株11株。70%以上菌株表现敏感的药物有丁胺卡那霉素、氟苯尼考、头孢唑啉、左氧氟沙星和壮观霉素,70%以上菌株表现耐受的药物有复方新诺明、卡那霉素、庆大霉素、恩诺沙星和氟哌酸。16株大肠杆菌分属于4个血清型,O78占56.3%,为主要血清型。毒力基因irp2、fyuA、fimC和papC的检出率分别为68.8%、87.5%、75%和100%。总结说明,从表现输卵管炎的种鸭体内共分离到16株大肠杆菌,O78是主要的血清型,首选壮观霉素和氟苯尼考等敏感药物进行治疗。

To analyze the pathogenicity, medicine sensitive, serotype and virulence associated genes of Escherichia coli （E. coli） strains from salpingitis ducks, the livers of ducks typically showing salpingitis were collected, from which the E. coli was isolated and identified. Pathogenicity of E. coli isolates were determined by subcutaneous injection of susceptible ducks. Serotype of E. coli isolates were detected by agglutination test. Virulence associated genes were detected by PCR. 16 strains of duck E. coli were identified, among which there were 11 virulent strains. More than 70% of the strains were sensitive to Amikacin, Florfenicol, Cefazolin, Levofloxacin and Spectinomycin. More than 70% of the strains were resistant to Trimethoprim sulfamethoxazole, Kanamycin, Gentamicin, Enrofloxacin and Ciprofloxacin. These strains were belonged to 4 serotypes, among which 078 was the main serotype which accounted for 56.3%. The detection rate of irp2 gene, fyuA gene, fimC gene and papC gene were 68.8%, 87.5%, 75%, 100%. 16 strains of E. coli were isolated from salpingitis ducks and 078 was the main serotype. Spectinomycin and Florfenicol were the suitable drugs.