HPLC测定番石榴叶乙酸乙酯提取物中5种黄酮苷的含量

Determination the 5 flavonoid glycosides of ethyl acetate extract from Psidium guajava leaves with HPLC

目的:建立番石榴叶提取物中金丝桃苷、异槲皮苷、瑞诺苷、槲皮素-3-O-β-D-吡喃阿拉伯糖苷、槲皮素-3-O-α-L-呋喃阿拉伯糖苷的多成分的含量测定方法。方法:色谱柱:S y n c r o n i s C18色谱柱(4.6mm×250mm,5μm),以乙腈-0.2%磷酸水梯度洗脱,流速1.0mL/min,检测波长254nm,柱温40℃。结果:金丝桃苷、异槲皮苷、瑞诺苷、槲皮素-3-O-β-D-吡喃阿拉伯糖苷、槲皮素-3-O-α-L-呋喃阿拉伯糖苷的线性范围为0.0318-0.2544μg(r=0.9998)、0.0575-0.4600μg(r=0.9998)、0.1860-1.4880μg(r=0.9998)、0.1482-1.1856μg(r=0.9998)、0.2012-1.6816μg(r=0.9998),平均加样回收率分别为100.7(RSD=2.6%),100.5(RSD=0.8%),103.4(RSD=2.6%),98.1(RSD=1.7%),100.7(RSD=1.2%),精密度、重复性、稳定性均良好。结论:所建立的方法准确可靠,重复性好,可用于番石榴叶提取物的含量测定。

Objective： To establish the quantitative analysis method for hyperin, isoquercitrin, reynoutrin, quercetin-3- O-β-D-arabinopyranoside, quercetin-3-O-α-L-furanarabino side ethyl acetate from Psidium guajava leaves extracts. Methods： High performance liquid chromatography （HPLC） was performed on a Syncronis C18 column （4.6mm×250mm, 5μm）. The acetonitrile with 0.2% phosphoric acid （v/v） was used as an aqueous solvent （A） and CH3CN as an organic solvent （B） with gradient at a flow rate of 1.0mL/min. The column temperature was set at 40℃, and UV detection wavelength was at 254nm. Results： The quantitative analysis by HPLC showed that the linear rang of hyperin, isoquercitrin, reynoutfin, quercetin-3-O-β- D-arabinopyranoside, quercetin-3-O-α-L-furanarabino side ethyl acetate was 0.0318-0.2544μg （r=0.9998）, 0.0575-0.4600μg （r=0.9998）, 0.1860-1.4880μg （r=0.9998）, 0.1482-1.1856 μg（r=0.9998）, 0.2012-1.6816μg （r=0.9998）, respectively. The average recovery of sample above was, in order, 100.7 （RSD=2.6%）, 100.5 （RSD=0.8%）, 103.4 （RSD=2.6%）,98.1 （RSD=1.7%）, 100.7 （RSD=1.2%）. Conclusion： The method was accurate, feasible and reliable, which was available for the quantitative analysis of ethyl acetate extract from Psidium guajava leaves.