肺癌细胞裂解物刺激树突状细胞诱导产生免疫应答的实验研究

Research of Lung cancer cell lysate stimulate on dendritic cells and induce immune response

目的采用肺癌细胞裂解物致敏树突状细胞(DCs)的方法制备肺癌疫苗并进一步通过实验验证其疗效。方法利用外周血的单个核细胞经粒细胞-巨嗜细胞集落刺激因子(GM-CSF)和人白细胞介素4(IL-4)体外诱导产生树突状细胞,用A549(人肺腺癌细胞系)细胞冻融抗原对其进行冲击诱导自体细胞毒T淋巴细胞(CTLs)产生。通过细胞毒试验及ELISA测定细胞因子的分泌和CTLs杀伤活性。制备荷瘤裸鼠模型,不同分组裸鼠经过一次或多次皮下注射CTLs。结果 A549冻融抗原致敏的DCs增加CTLs增殖,诱导的CTLs对A549细胞产生特异性杀伤,经过一次及多次接种后荷瘤裸鼠肿瘤生长减缓,并且肿瘤变小及生存时间较对照组明显延长,起到抑制肿瘤生长和延长生存时间的作用。结论通过实验证实了肺癌细胞裂解物抗原来刺激树突状细胞治疗肺癌A549荷瘤裸鼠模型是安全有效、可行的。为临床应用以树突状细胞为基础的肺癌疫苗免疫治疗提供了实验依据。

Objective The lung cancer cell lysates pulsed dendritic cells（DCs）are prepared for lung cancer vaccine and further verified the effect by experiments. Methods Using granulocyte macrophage colony stimulating factor（GM-CSF）and human interleukin 4（IL-4） ,mononuclear cells in peripheral blood were induced dendritic cells in vitro, A549 （human lung adenocarcinoma cell line）cell freeze-thaw antigen to the shock induced autologous cytotoxic T lymphocyte lymphocyte（CTLs）generation. Used cytotoxicity and ELISA to test secretion of CTLs cytokines and cytotoxic activity. Preparation of hearing cancer nude mice model, different groups gave one or multiple subcutaneous injection of CTLs. Results A549 freeze-thaw antigen sensitized DCs increased CTLs proliferation,induced CTLs specific cytotoxicity on A549 cells. After one or multiple inoculated in nude mice,cancer growth was slower,tumor size was smaller and survival time was prolonged compared with the control group,it played a role of inhibiting the growth of tumor and prolonging the survival time. Conclusion The experiments confirmed that using lung cancer cell lysate antigen to stimulate dendritic cells in the treatment of lung cancer A549 in nude mice model was safe, effective and feasible. It provides the experimental basis for clinical application of treatment of immune lung cancer vaccine based on dendritic cells.