钨系纳米颗粒与HepG2肿瘤细胞作用的瞬态吸收谱研究

Study on Transient Absorption Spectrum of Tungsten Nanoparticle with HepG2 Tumor Cell

利用M T T法研究了钨系纳米颗粒对 HepG2肝肿瘤细胞抑制作用，利用瞬态吸收谱（T AS ）技术的吸收发射谱表征肿瘤细胞和纳米粒子之间的电荷传递，讨论了钨系纳米颗粒在肿瘤疾病早期检测以及抗肿瘤特性。设计HepG2实验体系中，抗肿瘤活性的最佳药物浓度为100～150μg · mL -1，反应时间在6 h之内很强，在24 h之内已经基本完成。实验证实，瞬态吸收谱技术可以作为肿瘤的检测方法，及表征纳米生物传感器和肿瘤细胞之间的电荷传递，钨系纳米颗粒具有作为抗肿瘤药物的应用前景。

Significance of this study lies in tungsten nano materials can be used as a preliminary innovative medicines applied basic research .This paper investigated the inhibition of tungsten nanoparticles which effected on human hepatoma HepG 2 cells by MTT .The authors use transient absorption spectroscopy （TAS） technology absorption and emission spectra characterization of charge transfer between nanoparticles and tumor cell .The authors discussed the role of the tungsten nanoparticles in the tumor early detection of the disease and its anti-tumor properties .In the HepG2 experiments system ,100~150 μg · mL -1 is the best drug concentration of anti-tumor activity which recact violently within 6 hours and basically completed in 24 hours .The results showed that transient absorption spectroscopy can be used as tumor detection methods and characterization of charge transfer between nano-biosensors and tumor cells .Tungsten nanoparticles have potential applications as anticancer drugs .