非小细胞肺癌组织miR-10b表达临床意义分析

Analysis of clinical and pathological significance of expression of miR-10b in the non-small cell lung cancer

目的:探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)组织miR-10b的表达及其与临床病理因素的关系。方法:收集2005-01-01-2006-12-01中国医科大学附属第一医院病理科存档蜡块50例NSCLC和癌旁肺组织(距离癌组织>3cm),采用实时定量PCR检测NSCLC组织和相应癌旁组织中miR-10b的表达,分析miR-10b表达与临床病理参数的相关性,通过Kaplan-Meier进行生存分析。结果:50例NSCLC组织和相应癌旁组织中,34例癌组织miR-10b表达高于癌旁组织;癌组织miR-10b表达为1.55±0.57,相应癌旁组织为1.01±0.38,t=-2.182,P=0.034。miR-10b的表达水平与病理分期(U=189,P=0.010)和淋巴结转移(χ2=6.650,P=0.010)呈正相关,而与其他临床病理因素如性别、年龄、发病部位、病理分型和分化等均无相关性。miR-10b高表达的NSCLC患者5年生存率为4%(1/25),明显低于miR-10b低表达患者的32%(8/25),χ2=6.504,P=0.011。结论:miR-10b在NSCLC的发生发展中起着重要作用,可能成为NSCLC预后判断的指标,miRNA可能成为NSCLC分子诊断和靶向治疗的重要指标。

OBJECTIVE：To investigate the expression of MiR-10b in non-small-cell lung cancers and its correlation with clinical parameters. METHODS： Tumor specimens and adjacent normal tissues from 50 patients with NSCLC who underwent complete surgical resection were collected at First Affiliated Hospital of China Medical University from January 1st 2005 to December 1st 2006. The expressions of miR-10b in 50 NSCLC tissues and adjacent normal tissues were examined using RT-PCR. Kaplan-Meier analysis was used to compare the differences in the survival of subgroups of patients. RESULTS： In the 50 NSCLC specimens and normal lung tissues examined,miR-10b expression was higher in 34 cases of NSCLC （2-deha delta CT normal= 1.01±0.38,2-delta delta CT cancer= 1.55±0.57,t= -2. 182,P=0. 034）. Overexpression of miR-10b was correlated with advanced TNM stage （U= 189,P= 0. 010）and lymph node metastasis （χ^2= 6.65, P= 0. 010）. No correlation was observed .regarding gender, age, site of occurrence, histology or differentiation. The Kaplan-Meier analysis revealed that the 5-year survival rate was 4% （1/25） in patients with miR-10b overexpression and 32 % （8/25） in patients with low miR-10b expression, which was statistically significant （P= 0. 011）. CONCLUSIONS： The present study reported miR-10b expression pattern in NSCLC for the first time, suggesting miR-10b plays an important role in NSCLC development.