固本抑瘤Ⅲ号方联合吉西他滨对人胰腺癌裸鼠异位移植瘤的抑瘤作用

Effect of traditional Chinese medicine composit Guben Yiliu Ⅲ combined with gemcitabine on human pancreatic cancer xenograft in nude mice

目的探讨固本抑瘤Ⅲ号方以及与吉西他滨联合治疗人胰腺癌裸鼠异位移植瘤的抑瘤作用。方法将40只荷瘤裸鼠随机分为对照组、吉西他滨组、固本抑瘤Ⅲ号联合吉西他滨组、固本抑瘤Ⅲ号组,每组10只。于接种后第8天开始给药,观察指标为瘤重、裸鼠体重、移植瘤体积。结果吉西他滨组、固本抑瘤Ⅲ号联合吉西他滨组、固本抑瘤Ⅲ号组的抑瘤率分别为49.2%、68.9%和28.0%。固本抑瘤Ⅲ号联合吉西他滨组较吉西他滨组抑瘤作用更强(P<0.05)。吉西他滨组、固本抑瘤Ⅲ号联合吉西他滨组、固本抑瘤Ⅲ号组移植瘤体积均较对照组明显降低,差异有显著性。固本抑瘤Ⅲ号联合吉西他滨组体重下降明显,体重较对照组、吉西他滨组及固本抑瘤Ⅲ号组明显下降,差异有显著性。结论固本抑瘤Ⅲ号方具有增加吉西他滨化疗治疗人胰腺癌裸鼠腋下移植瘤疗效的作用。

Objective To explore whether the Chinese medicine Guben Yiliu Ⅲ can improve the effect of gemcit -abine on human pancreatic cancer xenograft in nude mice . Methods Nude mice with transplanted human pancreatic cancer were divided randomly into 4 groups： control group, gemcitabine treatment group , combined （ Guben Yiliu Ⅲ ＋gemcitabine） group, and Guben Yiliu Ⅲ group, 10 mice in each group.The gemcitabine group and combined group were treated with gemcitabine from the 8th day after transplantation in a dose of 100 mg/kg by i.p.injection, twice a week. Guben Yiliu Ⅲ and combined groups were given the aqueous solution of Guben Yiliu Ⅲ granules p .o.since the 8th day after transplantation .Result The inhibition rate of transplanted tumor in the three treatment groups were 48.9% in the gemcitabine group , 68.9%in the combined group , and 28.0%in the Guben Yiliu Ⅲ group .The combined group showed a significantly higher inhibition rate than the gemcitabine group （P〈0.05）.The gemcitabine group, combined group and Guben Yiliu Ⅲ group showed a significantly slower growth rate than the control group .However, the combined treatment group showed a pronounced side effect and body weight loss than the other 3 groups .Conclusions The Chinese medicine Guben Yiliu Ⅲ can improve the inhibitory effect of gemcitabine on nude mice with human pancreatic cancer xenograft in the auxilla of nude mice .