花生过敏患者血清中抗Arah1的IgG与IgE分离纯化研究

Purification of anti-Ara h1 antibody from peanut allergy serum

Ara h1是花生的一种主要致敏原蛋白,能够使花生过敏患者产生特异的IgG与IgE,从而导致花生过敏症状。Ara h1与其特异IgG和IgE的结合是导致花生致敏的一个重要原因。本研究利用Protein A亲和层析柱与Ara h1作为配体的亲和层析柱纯化抗Ara h1的IgG与IgE。高纯度的抗Ara h1特异IgG与IgE对探讨研究其与Ara h1之间相互作用具有重要意义。

Ara h1 is a major allergen proteins in peanut, which can make peanut allergic patients produce specific IgG and IgE. Ara hl and the specific binding of IgG and IcE is an important cause of peanut allergy. ProteinA affinity chromatography and affinity chromatography with Ara hl as a ligand were used to purify anti-Ara hl IgG and IcE. Purified specific IgG and IcE antibodies have important significance for the study of allergen and antybody interactions.