粉防己碱对人肺癌A549细胞血管生成拟态的影响

Effects of Tetrandrine on Vasculogenic Mimicry of Human Lung Cancer A549 Cell Line

目的:研究粉防己碱对人肺癌A549细胞血管生成拟态的影响。方法:采用细胞黏附试验观察粉防己碱对A549细胞黏附作用的影响;通过细胞迁移试验检测粉防己碱对A549细胞迁移能力的影响;通过血管生成拟态试验观察粉防己碱对A549细胞体外类血管生成的作用;实时定量聚合酶链反应(RT-PCR)法检测粉防己碱对A549细胞血管生成拟态相关基因表达的影响。结果:5、10、20μmol/L粉防己碱作用A549细胞30 min后,细胞的黏附作用受到抑制,并与浓度呈正相关;与对照比较,5、10、20μmol/L粉防己碱处理A549细胞12、24 h后,A549细胞迁移数减少,A549细胞管腔形成数明显减少,管腔长度明显缩短;A549细胞以粉防己碱处理后,血管生成拟态相关基因VE-cadherin、VEGF的表达减弱。结论:粉防己碱可抑制A549细胞黏附、细胞迁移和血管生成拟态,其机制可能与抑制血管生成拟态相关基因VE-cadherin、VEGF的表达有关。

OBJECTIVE： To study the effects of tetrandrin on vasculogenic mimicry of human lung cancer A549 cell line. METHODS： The effect of tetrandrin on the cell adhesion of A549 was observed by cell adhesion assay. The effect of tetrandrin on the cell migration of A549 was studied by cell scratch adhesion test. The effect of tetrandrin on the tube-like formation of A549 cell in vitro was observed by tube formation assay. The effect of tetrandrin on the expression of A549 vasculogenic mimicry related genes was assessed by semi-quantitative RT-PCR assay. RESULTS：5,10 and 20 μmol/L tetrandrin effectively inhibited the cell adhesion of A549 cell 30 min later and was positively associated with concentration. Compared with control solution,5, 10 and 20μmol/L tetrandrin strengthened the migration of A549 cell, inecreased the number of cell tube formulation significantly and shortened the length of cell tube 12 and 24 hours later. Tetrandrin markedly inhibited the expression of vasculogenic mimicry-related genes VE-cadherin and VEGF in A549 cell. CONCLUSIONS. Tetrandrin could effectively inhibit the cell adhension and cell migration of A549, and the A549-mediated vasculogenic mimicry, which may be associated with inhibiting the expression of vasculogenic mimicry-related genes VE-cadherin and VEGF.