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Sirt1在神经胶质瘤及瘤旁组织中的表达差异研究 被引量：1

The different expression of Sirt1 in the tissues of human glioma and para-glioma.
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摘要 目的研究神经胶质瘤及瘤旁组织中的沉默信息调节因子1(Sirt1)基因的表达差异。方法应用基因芯片技术检测30例Sirt1基因在神经胶质瘤及瘤旁组织的表达差异,随后应用RT-PCR及免疫组织化学实验验证并分析Sirt1基因的表达差异。结果基因芯片筛选出存在差异表达的基因,其中Sirt1基因在神经胶质瘤中的表达较瘤旁组织中的表达明显下降,RT-PCR实验证实与基因芯片结果一致,免疫组织化学实验结果与基因芯片、RT-PCR结果一致。结论Sirt1在神经胶质瘤及瘤旁组织中的表达存在明显差异,Sirt1基因的表达可能与神经胶质瘤的发生发展密切相关。 Objective To explore the changes in gene Sirtl expressions in tissues of human glioma and para - glioma. Methods A 44 000 gene cDNA microarray （Affymetfix） was used to examine gene expressions in the tissues of human glioma and para - glioma. The Sirtl genes were identified and subjected to real - time PCR analysis. Results The expression of Sirtl in tissues of glioma was extremely lower than that in tissues of para - glioma. The results of real - time PCR were consistent with those of genechip analysis. Conclusion The expressions of Sirtl were obviously different between tissues of glioma and para - glioma. Sirtl may be associated with carcinogenesis of glioma.
作者 陈旭 张锦前 刘藏 王月平 侯瑞光
机构地区 首都医科大学附属北京友谊医院神经外科 首都医科大学附属北京地坛医院传染病研究所
出处 《临床和实验医学杂志》 2014年第16期1332-1335,共4页 Journal of Clinical and Experimental Medicine
关键词 神经胶质瘤 沉默信息调节因子1(Sirt1) 实时荧光定量PCR 免疫组织化学 Glioma Sial RT-PCR Immunohistochemistry
分类号 R739.4 [医药卫生—肿瘤]
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参考文献19

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同被引文献21

  • 1熊敏,何青莲,汪建,邓恒山.Caspase-3在老年豚鼠耳蜗螺旋神经节细胞的表达[J].中国组织化学与细胞化学杂志,2006,15(5):547-549. 被引量：5
  • 2肖伯奎,华清泉,王勤瑛.正常大鼠听觉中枢四种氨基酸类神经递质测定[J].听力学及言语疾病杂志,2007,15(6):475-477. 被引量：5
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临床和实验医学杂志
2014年 第16期

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