黄金蒲桃对低温逆境的生理响应及其抗寒性

Physiological response to low temperature stress and evaluation of cold resistance of Xanthostemon chrysanthus

黄金蒲桃是华南等小部分地区引种栽培的特色园林树种,但低温寒害是影响其扩大引种栽培范围的主要因素之一。以重庆永川地区自然降温和模拟降温两个不同低温条件下的黄金蒲桃为材料,研究黄金蒲桃对低温逆境胁迫的生理响应。结果表明,在自然降温和模拟降温条件下,黄金蒲桃叶片的脯氨酸、可溶性蛋白、丙二醛(MDA)含量以及过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性均比对照增加,且在自然最低温度和低温胁迫时间延长时达到最大值。对自然降温和低温胁迫后并进行生长恢复试验的冷害指数进行观察发现,4℃低温处理72 h和在大棚自然越冬的冷害指数远低于室外露地大田栽培自然越冬,说明黄金蒲桃具有一定的抗寒性,基本可以在重庆永川地区安全越冬生长。

Xanthostemon chrysanthus is a characteristic landscape tree specie introduced and cultivated in South China,cold injury is one of the main factors which affecting its expansion of cultivated area.Taking X.chrysanthus under the conditions of natural cooling and artificial low temperature of Chongqing Yongchuan as materials,this study investigated the physiological response of X.chrysanthus to low temperature stress.The results showed that,the contents of proline,soluble protein,MDA and the activities of POD,SOD,CAT under natural cooling and artificial low temperature were higer than that of the contrast.The above indexes reached a maximum when the time of natural lowest temperature and artificial low temperature stress extended.The restoring growth test indicated that,the chilling injury indexes of seedling under 4℃ for 72 h and natural overwinter in greenhouse were much lower than that of field cultivation in open field.It proved that X.chrysanthus had some cold resistance ability,and it could over wintering safely in Chongqing Yongchuan district.