树脂法分离纯化山楂黄酮

Separation and purification of flavonoids extracted from hawthorn by macroporous resins

为确定大孔树脂对山楂黄酮的分离效果，比较了10种大孔吸附树脂对山楂黄酮的静态吸附效果，在确定了适合的大孔树脂后，通过进一步的动态吸附过程分析，确定D101树脂适合于山楂黄酮的吸附，其静态吸附率为88．1％，解吸率为92．9％。并对其动态吸附特性进行了研究，确定的最佳工艺参数为：进样黄酮浓度18．75mg／mL、pH3-4、上样流速1BV／h、洗脱液乙醇体积分数75％、洗脱液流速1BV／h；上样量达到6BV时，树脂达到吸附平衡，当洗脱液用量为3BV。达到洗脱终点。经D101树脂在最佳工艺条件下纯化后，所获得纯化产物中芦丁、金丝桃苷和槲皮素的总含量达到65．47％，回收率为86．22％。

in order to investigate the separation efficiency of macroporous resin on hawthorn fiavonoids,ten types of macroporous resins was used to study the static adsorption properties on hawthorn fiavonoids,after the optimum macroporous resins were deserved,D101 was seen as the optimal resin by the analysis of dynamic adsorption process of hawthorn flavonoids,the results indicate that static adsorption ratio of hawthorn flavonoids was 88.1%,desorption ratio was 92.9%. The optimized dynamic adsorption condition was hawthorn flavonoid concentration 18.75mg/mL,pH3-4,1oading rate 1BV/h,volume fraction of ethanol 75% ,adsorption ratio 1BV/h. The resin adsorption equilibrium was deserved when the sample volume was 6BV,and elution could be finished when the amount of eluent achieved 3BV. Under this condition,the purity of total concentration of rutin,hyperin and quercetin of hawthorn extract was 65.47%,and the recovery ratio was 86.22%.