肉桂单宁的提取工艺比较研究

Study on extraction technology of tannin from Cinnamon

进行了溶剂法和超声辅助法提取肉桂单宁的工艺研究，采用了儿茶素为标准品，香草醛-硫酸为显色剂的分光光度法定量分析肉桂单宁含量。结果表明，儿茶素浓度（C）与吸光度（A）在0．05—0．30mg／mL浓度范围内呈线性关系，方程为：Y=3．0153X＋0．0050。溶剂法提取的优化工艺为：乙醇体积分数60％，料液比l：30g／mL，提取时间150min，提取温度70℃，单宁得率为42．35mg／g。超声辅助法最佳工艺为：乙醇体积分数50％，料液比1：20g／mL，超声功率360W，提取时间40min，提取温度40℃，单宁得率为41．28mg／g。与溶剂法相比，超声辅助法显著减少了溶剂用量，降低了提取温度，缩短了提取时间。

The extraction process of tannin from Cinnamon was studied with the methods of solvent extraction and ultrasonic-assisted extraction. Using the quantitative analysis of tannin from Cinnamon with catechin as the standard and vanillin-sulfuric acid as chromogenic reagent to tracking tannin content in extraction process. The results showed that the absorbance had good linear relationship with the concentration of catechin in the range of 0.05-0.30mg/mL,the regression equation was Y=3.0153X＋0.0050. The optimal solvent extraction conditions were solvent of 60% ethanol,the ratio of material to solvent at 1：30g/mL,extraction time of 150rain, and temperature at 70℃ ,the extraction yield of tannin was 42.35mg/g. The optimal Ultrasonic-Assisted extraction conditions were solvent of 50% ethanol,the ratio of material to solvent at 1：20g/mL,the ultrasonic power at 360W, extraction time of 40min,and temperature at 40℃,the extraction yield of tannin was 41.28mg/g. Compared with the solvent extraction,the ultrasound-assisted extraction significantly decreased the dosage of solvent consumption, reduced the extraction temperature and had shorten the extraction time.