苹果多酚的固相萃取纯化研究

Study on solid phase extraction in the purification of apple polyphenols

研究了用固相萃取法纯化，高效液相色谱法测定苹果中多酚类物质的方法。以苹果冻干粉为原料，加标回收率为指标，从四种固相萃取小柱中筛选出分离效果较好的Waters Oasis HLB固相萃取小柱对提取液进行预处理。以phenomenex Luna C18色谱柱（150mm×4．6mm，5μm）N固定相，以0．1％的甲酸溶液（v／v）和乙腈为流动相梯度洗脱，紫外双波长扫描280、320nm条件下检测。结果：检测出七种中性多酚，三种酸性多酚。加标回收率为95％，104％，相对标准偏差均小于2．2％。结论：该方法快速、灵敏、简便、准确，杂质干扰少，10种多酚类物质峰分离度好。

A method of solid phase extraction combined with HPLC determination of polyphenols in apple was studied. Taking apple lyophilized powder as raw material,recovery of the polyphenols as the measure indicators,Waters Oasis HLB was screened out from four kinds of solid phase minicolumns to purify the apple extract. Gradient eluation was carried out on the chromatographic column phenomenex Luna C18（150mm×4.6mm,5μm）with the mobile phase consisted of 0.1% formic acid solution（v/v） and acetonitrile. Under these conditions,the components of apple polyphenols can be perfectly separated. An UV-detector was used for recording the chromatographic peaks of the apple polyphenols at 280nm and 320nm. The results showed that polyphenols in apple were seven kinds of neutral polyphenols and three kinds of acidic polyphenols,the recovery of the polyphenols was in the range of 95%-104% and RSD were lower than 2.2%. This method was rapid,sensitive,simple,accurate and less impurity interference,and ten kinds of polyphenols peaks were separated well.