大黄酚对单侧输尿管梗阻模型大鼠肾组织CTGF、α-SMA和FN的影响

Effect of chrysophanol on CTGF,α-SMA and FN of renal tissue in unilateral ureteral obstruction in rats

目的探讨大黄酚对大鼠单侧输尿管梗阻模型(UUO)肾间质纤维化的影响。方法 27只SD大鼠随机分为假手术组、模型组、大黄酚组。用单侧输尿管梗阻术建立肾间质纤维化模型,大黄酚治疗14 d后检测血肌酐、尿素氮水平;采用免疫组织化学方法观察肾组织结缔组织生长因子(CTGF)、α-平滑肌肌动蛋白(α-SMA)、纤维连接蛋白(FN)的表达情况。结果与假手术组相比,模型组血肌酐、尿素氮的水平以及CTGF、α-SMA、FN的表达均显著增高。与模型组相比,大黄酚组血肌酐、尿素氮水平降低,CTGF、α-SMA、FN的阳性表达显著下调。结论大黄酚可能通过抑制UUO大鼠肾组织CTGF、α-SMA、FN的表达,从而缓解肾间质纤维化的发生发展。

Objective To explore the effect of chrysophanol on rat renal interstitial fibrosis after unilateral ureteral obstruction（UUO）.Methods A total of 27 SD rats were randomly divided into 3 groups： a sham operation group,a UUO group and a chrysophanol group.Renal interstitial fibrosis model was established in rats with UUO.After the treatment for 14 days,the levels of Scr and BUN were measured.Expression of connective tissue growth factor（CTGF）,α smooth muscle actin（α-SMA） and fibronectin（FN） was shown by immunohistochemistry.Results The levels of Scr,BUN and the expression of CTGF,α-SMA and FN in the model group were significantly higher than those in the sham operation group.Compared with the model group,the levels of Scr,BUN and the expression of CTGF,α-SMA and FN in the chrysophanol group were obviously decreased.Conclusion Chrysophanol may prevent the progression of renal interstitial fibrosis via the downregulation of the expression of CTGF,α-SMA and FN.