铜绿假单胞菌注射液对非小细胞肺癌患者围手术期免疫水平的影响

Effect of perioperative administration of Pseudomonas aeruginosa injection on the immunological status of patients with non-small cell lung cancer

目的评价铜绿假单胞菌注射液(PA-MSHA)对非小细胞肺癌患者围手术期免疫功能的影响及安全性研究。方法将60例非小细胞肺癌患者随机分为PA-MSHA组(观察组,30例)和对照组(30例)。观察组均于手术结束前胸腔喷洒PA-MSHA 5 mL,术后第1、5、9日皮下注射PA-MSHA 1次,1 mL/次;对照组仅进行常规手术。2组患者均于手术前1 d,术后第2、6、10日采集外周静脉血,检测患者的各项免疫学指标,包括CD3+、CD4+、NK以及CD4+CD25+调节性T细胞(Treg)水平。结果观察组术后的CD3+、CD4+和NK均较术前显著升高(P<0.05),观察组CD4+CD25+Treg细胞比例较术前显著降低(P<0.05),而对照组的CD3+、CD4+和NK均较术前无显著变化(P>0.05),对照组CD4+CD25+Treg细胞比例较术前显著降低(P<0.05);观察组CD4+CD25+Treg细胞比例显著低于对照组(P<0.05)。结论铜绿假单胞菌注射液对非小细胞肺癌患者具有明显的免疫调节作用,安全性及耐受性良好。

Objective To determine the safety and effect of perioperative period administration of Pseudomonas Aeruginosa injection（PA-MSHA） on the immunological status of patients with non-small cell lung cancer（NSCLC）.Methods Sixty patients with NSCLC were randomly divided into an observation group and a control group.The patients in the observation group were sprayed PA-MSHA 5 mL into thoracic cavity at the end of the surgery.On day 1,5 and 9 after the operation,they were also subcutaneously injected PA-MSHA 1 mL qd.The patients in the control group were given conventional surgical treatment.Peripheral venous blood samples were taken 1 day before the operation,the next day,the 6th day and the 10th day after the operation,We m easured the immunological indexes of the patients,including the level of CD3＋,CD4＋,NK,and CD4＋CD25＋ regulatory T cells.ResultsThe values of CD3＋,CD4＋,and NK of the observation group after surgery were significant higher than those before the surgery（P 0.05）,and the values of CD4＋CD25＋ regulatory T cells in the observation group after the surgery were significantly lower than those before the surgery（P 0.05）.The values of CD3＋,CD4＋,and NK of the control group after the surgery were not significantly change（P0.05）,while the value of CD4＋CD25＋regulatory T cells of control group after the surgery was significantly lower than that before the surgery（P0.05）.The ratio of CD4＋CD25＋ regulatory T cells in the observation group was lower than that in the control group（P 0.05）.Conclusion PA-MSHA can enhance the perioperative immunological status of patients with NSCLC with satisfactory safety and tolerance.