肺动脉高压新生儿外周血中TNF-α、ANG-1和IL-8细胞因子的变化水平

Changes of TNF-α,ANG-1 and IL-8 levels in peripheral blood of neonates with pulmonary hypertension

目的:探讨肺动脉高压新生儿外周血中肿瘤坏死因子-α(TNF-α)、血管生成素-1(ANG-1)和白细胞介素-8(IL-8)细胞因子的变化水平与疾病的关系。方法:以临床确诊的42例肺动脉高压患儿为研究对象,与16例正常新生儿进行比较,采用Elisa方法检测血清中TNF-α、ANG-1和IL-8蛋白含量。采用实时荧光定量PCR法检测TNF-α、ANG-1和IL-8mRNA水平。结果:与健康新生儿相比较,肺动脉高压患儿外周血ANG-1的蛋白含量及ANG-1mRNA表达水平明显降低(P<0.01);TNF-α、IL-8的蛋白含量及TNF-α、IL-8的mRNA表达水平明显升高(P<0.01)。结论:TNF-α、ANG-1和IL-8参与肺动脉高压的发病过程。

Objective： To explore the relationship between changes of tumor necrosis factor - α （TNF - α）, angiopoietin - 1 （ANG - 1 ）, interleukin - 8 （ IL - 8） levels in peripheral blood of neonates with pulmonary hypertension and the disease. Methods. Forty -two neonates diagnosed as pulmonary hypertension definitely were selected as study objects, 16 normal neonates were selected as control group ; ELISA was used to detect the contents of TNF - α protein, ANG - 1 protein and IL - 8 protein in serum; real - time fluorescent quantitative PCR was used to detect the levels of TNF -α mRNA, ANG - 1 mRNA and IL - 8 mRNA. Results： Compared with control group, ANG - 1 protein content and ANG - 1 mRNA expression level in peripheral blood of neonates with pulmonary hypertension decreased significantly （ P 〈 0.01 ）, while the contents of TNF - α protein and IL - 8 protein and the expression levels of TNF - α mRNA and IL - 8 mRNA increased significantly （P 〈 0. 01 ） . Conclusion： TNF-α, ANG- 1 and IL- 8 are involved in the pathogenesis of pulmonary hypertension.