磷酸化p38 MAPK在小鼠卵泡发育中的表达

Expression of phosphorylated p38 MAPK during development of ovarian follicle in mice

目的:通过检测磷酸化p38 MAPK(p-p38MAPK)在小鼠卵泡发育过程中的表达,为研究丝裂原活化蛋白激酶(MAPKs)信号通路在卵泡生长、发育及成熟过程中的作用提供理论基础。方法:随机选取健康成年小鼠60只,按雌雄2∶1的比例合笼繁殖新生小鼠。取3、5、7、11及21天雌性健康小鼠(各30只)的卵巢组织,采用免疫组化SP法检测不同年龄段小鼠卵巢组织中p-p38MAPK蛋白的表达水平。结果:p-p38MAPK蛋白在各年龄段小鼠不同级别卵泡的卵母细胞胞浆中均有表达,在始基卵泡中表达最强,与各级卵泡相比,差异均有统计学意义(P<0.05);初级卵泡与次级卵泡比较,差异无统计学意义(P>0.05);初级卵泡、次级卵泡与窦状卵泡相比,差异均有统计学意义(P<0.05)。该蛋白在各级卵泡颗粒细胞中也均表达,始基卵泡与各级卵泡相比,差异均有统计学意义(P<0.05);初级卵泡与次级卵泡相比差异有统计学意义(P<0.05),而与窦状卵泡相比差异无统计学意义(P>0.05);在次级卵泡中表达最强,与各级卵泡相比差异均有统计学意义(P<0.05)。不同年龄段小鼠卵巢中,21天小鼠卵母细胞p-p38MAPK蛋白表达强度分别与3天和7天小鼠进行比较,差异均有统计学意义(P<0.05);3、5、7及11天各组相比,差异均无统计学意义(P>0.05)。各年龄组颗粒细胞p-p38MAPK蛋白表达强度相比,差异均有统计学意义(P<0.05)。结论:p-p38MAPK蛋白在小鼠卵泡发育过程中具有时空特异性,p-p38MAPK通路可能在卵泡发育过程中起重要的调控作用。

Objective： To provide a theoretical basis for researching the effect of mitogen -activated protein kinase （MAPK） sig- nal pathway in follicular growth, development and maturity by detecting the expression of phosphorylated p38 MAPK （ p -p38 MAPK） during development of ovarian follicle in mice. Methods： Sixty health adult mice were randomly selected and neonatal mice were obtained after mating according to the ratio of 2： 1. The ovarian tissue samples were obtained from healthy female mice at 3, 5, 7, 11 and 21 days （30 mice in each time point） ; immunohistochemical SP method was used to detect the expression levels of p -p38 MAPK protein in ovarian tis- sue of mice in different age groups. Results： p - p38 MAPK protein expressed in cytoplasm of oocytes in follicles of different degrees in mice in different age groups, the expression level of p - p38 MAPK protein in primordial follicles was the highest, compared with the other follicles of different degrees, there were statistically significant differences （ P 〈 0.05 ） ; but there was no statistically significant difference between primary follicles and secondary follicles （ P 〉 0. 05 ）, there were statistically significant differences between primary follicles, secondary folli- cles and antral follicles （ P 〈 0. 05） . p - p38 MAPK protein expressed in granulosa cells in follicles of different degrees, there were statistically significant differences between primordial follicles and the other follicles of different degrees （ P 〈 0. 05 ）, there was also statistically significant difference between primary follicles and secondary follicles （P 〈 0. 05）, but there was no statistically significant difference between primary follicles, secondary follicles and antral follicles （ P 〉 0.05 ） ; the expression level of p - p38 MAPK protein in secondary follicles was the highest, compared with the other follicles of different degrees, there were statistically significant differences （P 〈 0.05 ） . Among the mice in different age groups, there were statistically significant differences in expression level of p -p38 MAPK protein between 21 - day mice and 3 - day mice, 7 - day mice, respectively （ P 〈 0. 05 ）, but there was no statistically significant difference between 3 - day mice, 5 - day mice, 7 - day mice and 11 - day mice （ P 〉 0. 05） ; there was statistically significant difference in expression level of p, p38 MAPK protein in granulosa cells among mice of different age groups （ P 〈 0. 05 ） . Conclusion： p - p38 MAPK protein has spatio - temporal specificity dUring, development of ovarian follicle in mice, p - p38 MAPK signal pathway may play an important regulating role during follicular development.