反相高效液相色谱-激光诱导荧光法检测饮料中的维生素B_2

Detection of Vitamin B_2 Content in Beverages by RPHPLC-LIFD

在Sinochrom ODS—BP（5μm，4．6mm×200mm）色谱柱上，以甲醇-乙酸钠溶液为流动相，采用LIFD230激光诱导荧光检测器，建立了一种对饮料中维生素B：进行检测的反相高效液相色谱-激光诱导荧光检测方法。考察了样品处理方法、流动相配比及流动相pH对检测结果的影响。对检测条件进行了优化，在柱温为室温、流速为1mL／min、V（甲醇）：V（0．01mol／L乙酸钠溶液）为1：1及pH为5．0的条件下，维生素B2的线性范围为0．05—5μg／mL，样品加标回收率为96．52％～99．03％，RSD为3．25％，检出限为9．4×10^-4μg／mL。该方法具有灵敏度高、检出限低及响应快等特点。

In Sinochrem ODS-BP column （ 5 μ, 4. 6 mm × 200 mm） , a method was developed for the determination of vitamin B2 content in beverages by RP-HPLC with LIF detector, methanol-（0. 01 moL/L） NaAc as mobile phase. Sample processing method,mobile phase ratio and pH and so on were inversitigated. The testing conditions were optimized. The optimized chromatographic conditions were, column temperature, room temperature, flow rate, 1.0 mL/min, methanol ： （ 0.01 tool/L） NaAc = 1 ： 1 and pH were 5.0. Under the optimum conditions, the linear range for Vitamin B2 was 0. 05 - 5 g/mL, recoveries was 96. 52% - 99.03% , RSD was 3.25%, the detection limit was 9.4 × 10^-4 μg/mL. This method has the advantages of high sensitivity, low detection limit and fast response.