金雀异黄素对Ⅱ型胶原诱导性关节炎大鼠血清骨保护素及核因子-κB受体活化因子配体的影响

目的：研究金雀异黄素（genistein，Gen）对Ⅱ型胶原诱导性关节炎模型（typeⅡ，CIA）大鼠血清骨保护素（OPG）及核因子-κB 受体活化因子配体（RANKL）的影响。方法采用 SPSS 17.0软件随机抽样分为两组，编号1～10为空白对照组、11～60为造模组。造模组的50只大鼠建立CIA模型。造模成功的大鼠再按上述编号分为模型对照组、低剂量Gen组、中剂量Gen组、高剂量Gen组和甲氨蝶呤（MTX）组，于造模次日开始用药，各Gen组灌胃Gen混悬液1 ml/d；甲氨蝶呤组每周灌胃MTX 1 ml/次，浓度为0.1 mg/ml；空白组、模型对照组每天灌胃等量的生理盐水。用酶联免疫吸附试验（ELISA）检测各组大鼠血清RANKL及OPG的水平。结果骨代谢因子RANKL在模型对照组大鼠血清中表达量（0.328±0.044）的显著高于空白对照组（0.180±0.029）（P＜0.05）；Gen中、高剂量对RNAKL的抑制作用（分别为0.144±0.017、0.163±0.020）与甲氨蝶呤组（0.139±0.072）相当。模型对照组大鼠血清中的细胞因子OPG的表达水平（0.103±0.013）低于空白对照组（0.268±0.022）（P＜0.05）；Gen中、高剂量对OPG（分别为0.149±0.012、0.145±0.024）的上调作用与甲氨蝶呤组（0.160±0.056）相当。结论 Gen可影响 CIA 大鼠血清中 RANKL、OPG 水平，从而影响 OPG/RANKL 比值。提示 Gen 可能通过RANKL/OPG/RNAK通路抑制RA骨质破坏。

Objective To explore the effects of genistein（Gen） on OPG/RANKL in the serum of Collagen-induced arthritis（CIA） rats. Methods Sixty Sprague-Dawley rats were randomly divided into six groups, with 10 rats in each group respectively, normal group, model group, low-dose Gen treatment group, moderate-dose Gen treatment group, high-dose Gen treatment group and methotrexate group. The levels of OPG and RANKL were determined by ELISA. Results The level of RANKL serum was significantly higher in the model group （0.328±0.044） that in control group（0.328±0.044）, P〈0.05. Moderate-dose Gen treatment group and high-dose Gen treatment group can down-regulate of RANKL（0.144±0.017, 0.163±0.020）respectively. The level of OPG in the model group（0.103±0.013） was the lower than that in the control group（0.268±0.022）, P〈0.05. Gen treatment group and high-dose Gen treatment group can up-regulation of OPG（0.149±0.012, 0.145±0.024）respectively. Conclusion The Gen can enhance the OPG/RANKL ratio for the CIA rats, which may exert their inhibitory effect of RA bone destruction.