泻肝安神方对睡眠剥夺大鼠学习记忆行为及海马形态的影响

Effect of Xiegan Anshen Formula on learning-memory behavior and hippocampal morphology in rats after sleep deprivation

目的研究泻肝安神方对睡眠剥夺大鼠学习记忆行为及海马形态的影响。方法采用对氯苯丙氨酸腹腔注射法诱导大鼠睡眠剥夺模型,分别以不同剂量泻肝安神方及地西泮进行干预。观察大鼠的一般情况、逃逸潜伏期及穿越平台次数。采用HE染色,对各组大鼠海马进行形态学观察。结果泻肝安神方可延长睡眠剥夺模型大鼠的睡眠时间,降低逃逸潜伏期,增加穿越平台次数,减轻海马锥体层细胞损伤。结论泻肝安神方可提高睡眠剥夺大鼠受损学习记忆能力,改善海马组织形态。

Objective To research the effect of Xiegan Anshen Formula （XGAS） on learning-memory behavior and hippocampal morphology in rats after sleep deprivation. Methods Tablishment of rat model of insomnia was adopted by intraperitoneal injection with PCPA, then the rats were intervened with different dosage of XGAS and Western medicine to observe the general condition, the escape latency, times across the platform. Observed The hippocampal morphology was observed by HE staining. Results XGAS could prolong the sleep time of sleep deprived rats, reduce escape latency of rats, increase the times across the platform, reduce the hippoeampal pyramidal ceils injury. Conclusion XGAS could improve the impaired learning and memory ability of rats deprived of sleep, as well as the hippocampus morphology.