益肾化浊法对慢性脑缺血血管性痴呆大鼠学习记忆能力及海马缺血损害的影响

Effects of tonifying kidney and resolving turbidit method in learning and memory ability and hippocampal ischemic damage on chronic cerebral ischemia rats with vascular dementia

目的观察益肾化浊法对慢性脑缺血血管性痴呆大鼠学习记忆能力及海马缺血损害的影响。方法采用双侧颈总动脉永久性阻断法建立血管性痴呆大鼠模型,用聪圣颗粒对大鼠进行干预。水迷宫图像软件分析系统记录大鼠运动轨迹、逃避潜伏期、穿越平台次数及在各象限停留时间,HE染色观察大鼠脑组织海马病理形态学改变。结果与假手术组比较,模型组大鼠运动轨迹以边缘式和随机式为主,其逃避潜伏期延长(P<0.05),穿越平台次数减少(P<0.05),HE染色示海马神经细胞损伤严重。与模型组比较,聪圣颗粒中、高剂量组大鼠逃避潜伏期缩短(P<0.05),穿越平台次数增多(P<0.05),HE染色示海马神经细胞损伤明显减轻。结论益肾化浊法能够改善血管性痴呆大鼠的学习记忆能力,减轻海马组织的病理损伤。

Objective To observe the effects of tonifying kidney and resolving turhidit method in learning and memory ability and hippocampal ischemic damage on chronic cerebral ischemia rats with vascular dementia. Methods The bilateral carotid artery of rats were occluded to establish the model of permanent vascular dementia, then the rats were interevened by gavaging Congsheng Granules, afterwards the trajectory of rats, escape latency, the times across platform and time during platform quadrant were recorded by water maze image analysis software system. Furthermore, the hippocampal pathology morphology changes by HE staining were observed. Results Compared with the sham group, the trajectory of model group was given priority to edge type and random type, the escape latency was longer （ P 〈 0.05 ） , the number of crossing platform was reduced （ P 〈 0.05 ） and it was suggested that damage of hippocampal nerve cell was serious through HE staining. Compared with model group, the escape latent period of Congsheng Granules in high and medium dose groups was shorter （ P 〈 0. 05 ）, the number through platform was increased （ P 〈 0. 05 ） and it was suggested that damage of hippocampal nerve cell was reduced through HE staining. Conclusion The method of tonifying kidney and resolving turbidit could improve the ability of learning and memory in the rats with vascular dementia, and reduce the pathological damage of hippocampal tissue.