非离子表面活性剂-超声辅助萃取-高效液相色谱同时定金银花中绿原酸和咖啡酸的含量

Non-ionic Surfactant Extraction Solvent-based Ultrasonic-assisted Extraction Coupled with High Performance Liquid Chromatography for Sensitive Determination of Chlorogenic Acid and Caffeic Acid in Flos Lonicerae

目的：以3g/100mL非离子表面活性剂TritonX-100为萃取剂,建立一种简单快速的采用超声提取,高效液相色谱法测定金银花中绿原酸和咖啡酸的方法。方法：采用PhenomenexC18色谱柱（250mm×4.6mm,5μm）;流动相：甲醇-0.6%冰醋酸水溶液（25∶75,V/V）;流速：0.80mL/min;紫外检测波长：326nm;柱温：35℃。结果：该方法对绿原酸和咖啡酸分别在0.00076～190μg/mL（099995）,0.000864～216μg/mL（r=0.99966）范围内线性关系良好,定性检测限（S/N=3）依次为：0.029ng/mL,0.030ng/mL,样品回收率为95.13%～100.24%。结论：该方法具有样品前处理简单、绿色环保、灵敏快速等优点,可为金银花中绿原酸和咖啡酸的检测提供一个有效的方法。

Objective ： An HPLC method for the simultaneous determination of Chlorogenic acid and Caffeic acid in Flos Lonicerae was proposed based on rapid ultrasonic - assisted extraction using the non - ionic surfactant TritonX - 100 as the extractant. Methods ： The separation was performed on Phenomenex C18 （ 250 mm × 4.6 mm ,5 μm） column with detection wavelength of 326 nm. The mobile phase consisted of methanol -0.6% acetic acid solution （25：75 ,V/V） with the flow rate of 0.80 mL/min, and the column temperature was set at 35℃. Result：The calibration curves for Chlorogenic acid and Caffeic acid were liner between 0. 00076 - 190.00 μg/mL （ r = 0.99995 ）, 0. 000864 ~ 216. 00 μg/mL （ r = 0. 99966） respectively. The limit of detection （LOD） were 0. 029 ng/mL,0. 030 ng/mL, respectively. The recoveries of the two compounds range were 95.13 % - 100.24%. Conclusion ：The method was simple, rapid, convenient, sensitive, and environment -friendly, and thus can provide new and scientific means for assaying the contents of Chlorogenic acid and Caffeic acid in Flos Lonicerae.