茶黄素对葡萄糖诱导的人晶状体上皮细胞一氧化氮合成的影响

Effect of TFDG on synthesis of NO in human lens epithelial cells by high glucose

为探明茶黄素–3,3’–双没食子酸酯（TFDG）对人晶状体上皮细胞SRA01/04（Human lens epithelial cells,HLECs SRA01/04）的保护作用,采用MTT法建立高浓度葡萄糖（HG）诱导的HLECs损伤模型,同时观察HLECs细胞形态的变化。用不同浓度（4、8、16、64、32、64、128、256、512μmol/L）TFDG处理HLECs损伤细胞,采用化学比色法和硝酸还原法测定HLECs内一氧化氮（NO）的含量和一氧化氮合酶（NOS）的活性。结果表明：高浓度葡萄糖可以抑制人晶状体上皮细胞生长,用于HLECs损伤模型诱导的最佳葡萄糖浓度为50 mmol/L,最佳诱导时间为24 h；与未经处理的模型细胞相比,经浓度为16～64μmol/L TFDG处理的模型细胞的存活率显著提高（P〈0.05）；各个浓度的TFDG能极显著降低模型细胞内NOS的活性,极显著减少NO的合成（P〈0.01）,且呈浓度依赖性。以上结果表明,TFDG对高浓度葡萄糖诱导的HLECs细胞损伤具有较好的保护作用。

To investigate the effect of theaflavin-3, 3＇-digallate（TFDG） on human lens epithelial cells（HLECs SRA01/04）, MTT method was used to establish the oxidative damage model of HLECs induced by high concentration of glucose, at the same time, the changes of cell morphology was observed. The HLECs induced by high glucose were interfered with different concentrations of TFDG（4, 8, 16, 64, 32, 64, 128, 256, 512 μmol/L）, the NOS activity and NO level in HLECs were detected by chemical colorimetry and nitric acid reductase method respectively. It was found that HG can inhibit the growth of human lens epithelial cells. The optimal concentration of glucose and the optimal treating time are 50 mmol/L and 24 h. Compared with the model cells, survival rate of modle cells treated with TFDG（16–64 μmol/L） increased（P〈0.05）, and the NOS activity and NO synthesis in TFDG treated model cells were extremely significantly decreased（P〈0.01） in a dose dependent manner. In conclusion, TFDG showed good protect against damage of HLECs induced by high glucose.