富氢液对大鼠神经病理性痛的影响

Effect of hydrogen-rich saline on neuropathic pain in rats

目的 评价富氢液对大鼠神经病理性痛的影响.方法 健康雄性SD大鼠120只,体重200 ～ 250 g,采用随机数字表法,将其分为4组（n=30）：假手术组（S组）、富氢液组（H组）、坐骨神经慢性压迫性损伤组（CCI组）和CCI＋富氢液组（CH组）.CCI组和CH组采用CCI法制备大鼠神经病理性痛模型.H组和CH组于CCI术后1d开始腹腔注射富氢液10 ml/kg（0.6 mmol/L）,2次/d,连续3d.分别于CCI术前1d和术后3、5和7d,测定机械刺激缩足阈（PWT）和热刺激缩足潜伏期（PWL）;分别于术后3、5和7d,取术侧脊髓L4-6节段,采用ELISA法测定脊髓组织TNF-α、IL-1β、IL-6和高迁移率族蛋白B1（HMGB1）的含量,采用可见分光光度法测定MDA含量、SOD和过氧化氢酶（CAT）的活性.结果 与S组比较,CCI组和CH组CCI术后各时点PWT降低,PWL缩短,脊髓组织TNF-α、IL-1ββ、IL-6、HMGB1和MDA的含量升高,SOD和CAT的活性降低（P＜0.05）;与CCI组比较,CH组CCI术后各时点PWT升高,PWL延长,脊髓组织TNF-α、IL-1β、IL-6、HMGB1和MDA的含量降低,SOD和CAT的活性升高（P＜0.05）.结论 富氢液可减轻大鼠神经病理性痛,其机制与减轻炎性反应和脂质过氧化反应有关.

Objective To evaluate the effect of hydrogen-rich saline on neuropathic pain in rats.Methods One hundred and twenty male Sprague-Dawley rats,weighing 200-250 g,were randomly divided into 4 groups （n =30 each） using a random number table：sham operation group （S group）; hydrogen-rich saline group （H group）; chronic constrictive injury （CCI） group; CCI ＋ hydrogen-rich saline group （CH group）.Neuropathic pain was induced by CCI.The animals were anesthetized with intraperitoneal 10% chloral hydrate 300 mg/kg.The left sciatic nerve was exposed and 4 ligatures were placed on the sciatic nerve at 1 mm intervals.In H and CH groups,hydrogen-rich saline 10 ml/kg was injected intraperitoneally twice a day for 3 consecutive days starting from 1 day after CCI.The paw withdrawal threshold to mechanical stimulation with yon Frey filament （PWT） and paw withdrawal latency to thermal stimulation （PWL） were measured on the day before CCI （T0） and on 3,5 and 7 days after CCI （T1-3）.The rats were sacrificed after the last measurement of pain threshold at T4.The left lumbar segments （L4-6） of the spinal cord were removed for determination of the contents of tumor necrosis factor-alpha （TNF-α）,interleukin-1β （IL-1β）,interleukin-6（IL-6） and high-mobility group box-1 （HMGB1） （by ELISA）,and malondialdehyde （MDA） content and superoxide dismutase （SOD） and catalase （CAT） activities （using visible spectrophotometer）.Results Compared with S group,PWT was significantly decreased,PWL was shortened,the contents of TNF-α,IL-1β,IL-6,HMGB1 and MDA were increased,and the activities of SOD and CAT were decreased at each time point after CCI in CCI and CH groups.Compared with CCI group,PWT was significantly increased,PWL was prolonged,the contents of TNF-α,IL-1β,IL-6,HMGB1 and MDA were decreased,and the activities of SOD and CAT were increased at all the time points after CCI in group CH.Conclusion Hydrogen-rich saline can alleviate neuropathic pain in rats via reducing the inflammatory response and lipid peroxidation response.