七氟醚预先给药对大鼠内毒素性急性肺损伤时白细胞介素17表达的影响

Effects of sevoflurane pretreatment on expression of interleukin-17 in rats with lipopolysaccharide-induced acute lung injury

目的 评价七氟醚预先给药对内毒素性急性肺损伤大鼠白细胞介素17（IL-17）表达的影响.方法 健康成年雄性SD大鼠32只,体重220 ～ 260 g,2月龄,采用随机数字表法,将其分为4组（n=8）：对照组（C组）、七氟醚组（S组）、内毒素组（L组）和七氟醚＋内毒素组（SL组）.L组和SL组采用气管内滴注脂多糖（LPS）5 mg/kg的方法制备内毒素性急性肺损伤模型,其它2组给予等容量生理盐水,S组与SL组分别吸入2.4％七氟醚30 min时给予生理盐水或LPS.给予LPS后12 h处死大鼠,进行左侧支气管肺泡灌洗,进行支气管肺泡灌洗液（BALF）白细胞（WBC）和中性粒细胞（PMN）的计数.取右侧肺组织,光镜下观察病理学结果,并测定IL-17 mRNA及其蛋白表达.结果 与C组比较,L组和SL组BALF中WBC和PMN计数升高,肺组织IL-17 mRNA及蛋白表达上调（P＜0.01）;与L组比较,SL组BALF中WBC和PMN计数降低,肺组织IL-17 mRNA及其蛋白表达下调（P＜0.05或0.01）;SL组肺组织损伤明显轻于L组.结论 七氟醚预先给药可减轻大鼠内毒索性急性肺损伤时炎性反应,其机制与下调肺组织IL-17表达有关.

Objective To evaluate the effects of sevoflurane pretreatment on the expression of interleukin-17 （IL-17） in rats with lipopolysaccharide （LPS）-induced acute lung injury.Methods Thirty-two adult male Sprague-Dawley rats,weighing 220-260 g,aged 2 months,were randomly divided into 4 groups （n =8 each）：control group （group C）,sevoflurane group （group S）,LPS group （group L） and sevoflurane ＋ LPS group （group SL）.ALI was induced by slow intra-tracheal instillation of LPS 5 mg/kg in L and SL groups,while the equal volume of normal saline was administered in the same way in the other two groups.The rats inhaled 2.4 % sevoflurane for 30 min,and then normal saline and LPS were given in S and SL groups,respectively.The rats were sacrificed at 12 h after administration of LPS.The left main bronchus was lavaged and broncho-alveolar lavage fluid （BALF） was collected for determination of white blood cell （WBC） and polymorphonuclear leukocyte （PMN） counts.The right lung was removed for microscopic examination by using light microscope and for determination of -L-17 mRNA and protein expression in the right lung tissues.Results Compared with group C,WBC and PMN counts were significantly increased,and IL-17 mRNA and protein expression was up-regulated in L and SL groups.Compared with group L,WBC and PMN counts were significantly decreased,and IL-17 mRNA and protein expression was down-regulated in group SL.The pathological changes of lungs were significantly mitigated in group SL as compared with group L.Conclusion Sevoflurane pretreatment can reduce inflammatory reponses in rats with LPS-induced acute lung injury,and down-regulation of IL-17 expression in lung tissues is involved in the mechanism.