期刊文献+

吉兰-巴雷综合征源空肠弯曲菌致急性周围神经病Hartley豚鼠模型初步建立 被引量:1

Establishment of the model of Hartley guinea pigs with peripheral nerve disease induced by Guilliain-Barre syndrome campylobacter jejuni
下载PDF
导出
摘要 目的初步建立吉兰-巴雷综合征(Guilliain-Barre syndrome,GBS)源空肠弯曲菌感染致急性周围神经病Hartley豚鼠模型。方法以GBS患者分离的空肠弯曲菌经微需氧培养后制备菌悬液灌喂豚鼠。自灌喂当日起每日观察豚鼠的临床症状并分别对出现肢体活动障碍及灌胃后2周、3周、4周、5周的豚鼠进行取材,取脊神经根、坐骨神经及臂丛神经,进行苏木精-伊红染色(HE染色)、锇酸染色、免疫组织化学进行神经病理观察。结果空肠弯曲菌自然感染豚鼠后,豚鼠于5周内出现活动减少,食欲减退,体质量减轻症状;分别于2周、3周、4周出现神经病理改变,表现为HE染色可见神经纤维结构多样,轴索肿胀。免疫组织化学染色:鼠抗人神经细丝蛋白单克隆抗体示神经丝粗细不等,节段性萎缩;兔抗人髓磷脂碱性蛋白:髓鞘厚度均一,未见脱髓鞘样改变。锇酸染色:可见神经皱缩、髓鞘塌陷。结论吉兰-巴雷综合征源空肠弯曲菌口服灌喂感染Hartley豚鼠可导致急性周围神经病;Hartley豚鼠可以作为GBS的研究模型动物。 Objective To establish the model of Hartley guinea pigs with peripheral nerve disease induced by Guillain-Barre syndrome(GBS)-campylobacter jejuni.Methods Campylobacter jejuni isolated from the feces of GBS patients were fed guinea pigs after being micro-aerobic cultured.Since that day,the clinical symptoms of guinea pigs were observed daily,and guinea pigs with the limb movement disorder and gavage for 2weeks,3weeks,4weeks,5weeks were collected,in which the collected root of spinal nerves,sciatic nerve and brachial plexus were pathologically observed in hematoxylin eosin staining(HE staining),osmic acid staining and immunohistochemistry.Results The guinea pigs naturally infected with Campylobacter jejuni had symptoms of less activity,decreased appetite and body mass loss;the guinea pigs had nerve pathological changes for 2week,3weeks,4weeks,which manifested as the diversity of nerve fiber structure and swelling of axon with HE staining.Immunohistochemical staining with mouse anti human neurofilament monoclonal antibody of neurofilament showed different thickness and segmental atrophy;rabbit anti human myelin basic protein showed uniformity of myelin sheath thickness,but there was no demyelination.Osmic acid staining showed that the nerve was shrinking and myelin was collapsed.Conclusion The oral feeding of GBScampylobacter jejuni to Hartley guinea pigs could cause acute peripheral neuropathy syndrome;Hartley guinea pig can be used as animal model for the study of GBS.
出处 《临床荟萃》 CAS 2014年第8期896-899,F0002,共5页 Clinical Focus
基金 国家自然科学基金资助项目(81072481 30471919) 863计划资助项目(2006AA02A237)
关键词 格林-巴利综合征 空肠弯曲杆菌 周围神经系统疾病 豚鼠 Guillain-Barre syndrome campylobacter jejuni peripheral nervous system diseases guinea pigs
  • 相关文献

参考文献3

二级参考文献26

  • 1李春岩,薛平,刘瑞春,田文强.空肠弯曲菌致鸡格林─巴利综合征动物模型的初步研究[J].脑与神经疾病杂志,1994,2(2):68-69. 被引量:9
  • 2刘怀军,陈薇,刘瑞春,李春岩,李林芳,王勇.空肠弯曲菌感染后中枢神经系统病变动物模型的初步研究[J].脑与神经疾病杂志,2005,13(1):12-15. 被引量:5
  • 3王太一 韩子玉.实验动物解剖图谱[M].沈阳:辽宁科技出版社,2000.17-40.
  • 4陈志春 段晓波 刘垄荣.介绍一种豚鼠灌胃投药法.中国药理学通报,1986,2(4):54-54.
  • 5孙敬芳.动物实验方法学[M].北京:人民卫生出版社,2001.195.
  • 6Ursla Fernando,Debabrata Biswas,Brenda Allan,Sam Attah-Poku,Philip Willson,Alfonso Valdivieso-Garcia,Andrew A. Potter.Serological assessment of synthetic peptides of Campylobacter jejuni NCTC11168 FlaA protein using antibodies against multiple serotypes[J]. Medical Microbiology and Immunology . 2008 (1)
  • 7Carrillo C D,,Taboada E,Nash J H,et al.Genome-wide expres-sion analyses ofCampylobacter jejuniNCTC11168 reveals coor-dinate regulation of motility and virulence by flhA. Journal of Biolchemistics . 2004
  • 8Cordwell S J,Len A C,Touma R G,et al.Identification of mem-brane-associated proteins fromCampylobacter jejunistrains u-sing complementary proteomics technologies. Proteomics . 2008
  • 9Hartley-Tassell L E,Shewell L K,Day C J,et al.Identificationand characterization of the aspartate chemosensory receptor ofCampylobacter jejuni. Molecular Microbiology . 2010
  • 10Pei Z,Burucoa C,Grignon B,et al.Mutation in the peb1A locusofCampylobacter jejunireduces interactions with epithelial cellsand intestinal colonization of mice. Infection and Immunity . 1998

共引文献16

同被引文献7

引证文献1

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部