吲哚青绿介导光动力对防治兔眼后发性白内障的影响

The Impact on the Prevention of Posterior Capsule Opacification of Photodynamic Therapy with Indocyanine Green in Rabbits

目的通过比较观察吲哚青绿联合及不联合光照对兔眼后发性白内障发生的抑制作用,研究光动力在吲哚青绿防治兔眼后发性白内障形成中的作用.方法 24只兔眼随机分为ICG+光照组、ICG组、空白对照组,分别行晶状体超声乳化及皮质吸出术,ICG+光照组、ICG组在皮质吸出后前房内注入0.5 mL浓度为5 mg/mL的ICG;ICG+光照组用810 nm的半导体激光对囊膜进行低能量(50 mW/cm)照射2 min;空白对照组不进行前房内ICG注射及光照处理.术后3个月观察各组后囊膜的混浊程度及囊膜中晶状体上皮细胞增殖细胞核抗原(PCNA)的表达.结果 ICG+光照组的晶状体后囊膜混浊程度EPCO值与ICG组、空白对照组比较,差异有统计学意义(P<0.01),ICG组与空白对照组比较差异无统计学意义(P>0.05);囊膜的晶状体上皮细胞增殖核抗原增殖指数ICG+光照组与ICG组、空白对照组比较P<0.01,差异有统计学意义,ICG组与空白对照组比较差异无统计学意义(P>0.05).结论单纯ICG对晶状体上皮细胞没有明显抑制作用,ICG对晶状体上皮细胞的抑制作用是通过其作为光敏剂产生的光化学效应而实现的.

Objective To study the role of photodynamic therapy in prevention of posterior capsule opacification by observing the inhibition of indocyanine green on prevention of posterior capsule opacification in rabbits. Methods 24 rabbit＇s eyes were randomly divided in 3groups. Extracapsular lens extraction by phaeoemulsification was performed on 24 eyes. 0.5 ml ICG （5mg/ml） was injected in the capsular bag and the eye was illuminated with a diode laser （wavelength 810 nm） for 2min in one group; another group was injected only in the capsular bag with 0.5 mL ICG （5 mg/mL） ; The third group was not injected with ICG in the capsular bag and with no illumination by diode laser. After 3 months, we observed the degree of posterior capsular opacification and proliferation cell nuclear antigen expression in capsular lens epithelial cell. Results 3 months after surgery, there were statistically significant differences in posterior capsular opacification degree EPCO value in ICG combined with laser irradiation group, as compared with ICG group and blank control group （P〈0.05） , but there was no statistically significant difference between ICG group and the control group （P〉0.05） . There were statistically significant differences in the proliferation index of lens epithelial cell proliferation nuclear antigen in ICG combined with laser irradiation group, as compared with ICG group and blank control group （P〈0.01） , but there was no statistically significant difference between ICG group and the control group （P〉0.05） . Conclusion ICG alone has no inhibitory effect on lens epithelial cells, but ICG can induce photochemical effects as a photosensitizer, resulting in inhibition of lens epithelial cells.