非接触消毒方法对生物材料表面细菌生物膜的清除效果评价

The Assessment of Non-touch Disinfection Methods in Removing Bacterial Biofilm on the Surface of Biomaterial

目的评价非接触消毒方法对生物材料表面细菌生物膜的清除效果.方法将灭菌消毒后的PVC材料与表皮葡萄球菌RP62A混合培养48 h后,取出PVC材料分别行不同时间的紫外线、环氧乙烷消毒,扫描电镜观察生物膜的变化.用无菌生理盐水超声冲洗PVC材料,收集冲洗液接种于平板培养基,恒温培养,24 h后菌落计数法测定细菌菌落数.结果环氧乙烷组中消毒40 min、50 min、60 min与对照组菌落数(CFU/mL)分别为1 026、162、0、5 020,组间比较差异具有统计学意义(P<0.05).紫外线组中消毒30 min、40 min、50 min与对照组菌落数(CFU/mL)分别为266、48、0、5 020,组间比较差异有统计学意义(P<0.05).在环氧乙烷与紫外线组经过灭菌处理后,PVC材料表面细菌及生物膜细菌间胞外基质,呈片状残留,无生物膜形成,2组间差异不明显.结论环氧乙烷与紫外线法消毒对生物材料表面细菌生物膜都具有清除效果,且消毒时间越长效果越好.

Objective To assess the effects of different disinfection methods on removing bacterial biofilm on the surface of biomaterial. Methods The P^C material and epidermis staphylococcus RP62A were co-cultured for 48 hours, then the PVC material was disinfected with ethylene oxide and ultraviolet ray for different times. SEM was used to observe the changes in biofilm. The PVC material was was flushed with sterile saline, the washing fluids were collected, and cultured in plate culture medium, then was incubated in constant temperature for 24 hours, the colony counts was performed. Results In the ethylene oxide group, after 40 min, 50 min and 60min disinfection, the CFU was 1026, 162, 0 and 5020 in control group respectively, the difference between groups had statistical significance. In the ultraviolet ray group after 30 min, 40 min and 50 min disinfection, the CFU was 266, 48, 0 and 5020 in control group respectively, the difference between groups had statistical significance. After disinfection by ethylene oxide and ultraviolet ray, the SEM showed Extracellular matrix between bacteria and bacteria biofilm, plate shaped residues on the surface of PVC biomaterial, and no bioflim was formed. The difference between groups had no statistical significance. Conclusion method has good effect on removing bacterial biofilm of biological material with the longer disinfection time. Epoxy ethane and uhraviolet disinfection surface, and the better result can be got with the longer disinfection time.