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瘦素对动脉粥样硬化血管内皮细胞中IL-6表达的影响 被引量：3

Effect of Leptin on Interleukin-6 Expression in Vascular Endothelial Cells
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摘要 目的探讨瘦素在血管内皮细胞(VECs)对白细胞介素6(IL-6)表达的影响.方法采用原代细胞培养方法建立大鼠VECs细胞模型;100μmol/L瘦素刺激内皮细胞0,1,3,6,12 h.使用ELISA法检测各个分组细胞上清液IL-6的表达,real-time PCR检测IL-6 mRNA的相对表达量.结果与空白对照组相比,瘦素刺激组IL-6的表达高于对照组(P<0.05),且随着瘦素作用时间的延长IL-6蛋白及IL-6 mRNA的表达也随之增加(P<0.05).结论瘦素时间依赖性诱导血管内皮细胞IL-6的表达. Objective To investigate the effect of leptin on the expression of Interleukin 6 in vascular endothelial cells.Methods Vascular endothelial cells were culture by primary cell culture,100 umol/L leptin was used to stimulate endothelial cells for 0,1,3,6,12h.Then ELISA method was used to detect the expression of IL-6 protein,and the expression of IL-6 mRNA in vascular endothelial cells was detected with real-time fluorescence quantitative polymerase chain reaction （real-time PCR）.Results Compared with the blank control group,the expression levels of IL-6 were higher in the leptin stimulation group,and the expression levels of IL-6 protein and mRNA were increased when the action time extended.Conclusion Leptin can increase the expression and secretion of IL-6 in vascular endothelial cells in time-dependent manner.
作者 曹蕊 王先梅
机构地区 成都军区昆明总医院心血管内科
出处 《昆明医科大学学报》 CAS 2014年第8期26-29,共4页 Journal of Kunming Medical University
关键词 动脉粥样硬化 瘦素 白细胞介素6 Atherosclerosis Leptin IL-6
分类号 R541.4 [医药卫生—心血管疾病]
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参考文献10

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二级参考文献43

共引文献109

同被引文献27

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昆明医科大学学报
2014年 第8期

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