人参皂苷Rg1对AD大鼠脑片模型Bcl-2表达的影响

Effect of Ginsenoside Rg1 on Bcl-2 Expression in Rat Brain Slices Model of AD

目的观察人参皂苷Rg1对冈田酸(okadaic acid,OA)诱导的AD大鼠脑片模型Bcl-2表达的影响.方法将5周龄SD大鼠脑片随机分为对照组、模型组和小、中、大剂量人参皂苷Rg1组,每组10张脑片.各组脑片首先至于人工脑脊液中不做任何处理恢复1 h;然后Rg1小、中、大剂量组分别加入Rg1致使其终浓度为60、120、240μmol/L,培养至2 h;然后将OA加入模型组和人参皂苷R1各组中,致使其终浓度分别为1μmol/L,作用3 h,空白对照组不加任何处理.干预结束后,采用Westorn blot技术检测各组大鼠脑片Bcl-2蛋白表达水平.结果与对照组比较,模型组大鼠脑片Bcl-2的表达差异有统计学意义(P<0.01);与模型组比较,3个剂量人参皂苷Rg1组Bcl-2表达差异有统计学意义(P<0.01),其中以大剂量人参皂苷Rg1组Bcl-2表达水平最高.结论人参皂苷Rg1可能通过上调Bcl-2基因表达途径从而抑制神经元凋亡,对AD发挥神经保护作用.

Objective The purpose of this study was to investigate the effect of ginsenoside Rg1 on the Bcl-2 expression in rat brain slices model of AD induced by okadaic acid （OA）.Methods The brain slices of 5 week old SD rats were randomly divided into control group,model group,and ginsenoside Rg1 groups （including small,middle and large dose groups）,each group of 10 brain slices.Firstly,each brain slice was put in artificial cerebrospinal fluid without any treatment to restore 1 h.Then,Rg1 was added in the small,middle and large dose groups to make the Rg1 final concentration of 60,120 and 240 μ mol/L for 2 h,respectively.Lastly,OA was added in the model group and ginsenoside R1 groups to make the final concentration of 1 μ mol/L for 3 h.While the blank control group was not given any treatment.After intervention,the expression of Bcl-2 protein in the brain slices was detected using the Western blot technology.Results Compared with the control group,the expression levels of Bcl-2 in the brain slices of model group was decreased significantly （P＜0.01）.Compared with the model group,the expression levels of Bcl-2 in the three ginsenoside Rg1 groups were significantly increased （P＜0.01）,of which the Bcl-2 expression levels in the large dose ginsenoside Rg1 group were the highest.Conclusion Ginsenoside Rg1 could inhibit neuronal apoptosis and have the neuroprotective effect on AD by up-regulating the Bcl-2 gene expression.