急性白血病患者外周血中CECs、VEGF-C、VEGFR-2的变化及其临床意义

The Change and Significance of CECs, VEGF-C, VEGFR-2,3 in Peripheral Blood of Patients with Acute Leukemia

目的探讨循环内皮细胞(CECs)在急性白血病(AL)患者外周血中的数量变化及血清中血管内皮生长因子-C(VEGF-C)及其受体2(VEGFR-2)水平的变化及其临床意义.方法采用流式细胞仪(FCM)检测66例AL患者CECs的相对计数.采用ELISA的方法检测66例患者血清中VEGF-C、VEGFR-2的水平,并动态观察43例AL患者治疗前后的变化.结果 (1)AL患者初治组、CR组、NR组CECs外周血相对计数较对照组明显增高(P<0.05);CR组CECs相对计数较NR组明显减低(P<0.05);(2)AL患者初治组血清VEGF-C及VEGFR-2水平较对照组升高,2组差异有统计学意义(P<0.01);治疗后患者血清VEGF-C及VEGFR-2水平较治疗前降低,2组差异有统计学意义(P<0.01).结论 AL患者初治组外周血中CECs相对计数明显增高,治疗后CR组较NR组相对计数减降低;AL患者初治组血清中VEGF-C、VEGFR-2水平显著增高.CECs、VEGF-C、VEGFR-2其水平可能与AL患者病情发展、疗效及预后有一定关系.

Objectives To explore the change and clinical significance of circulating endothelial cells （CECs） in the peripheral vascular as well as ascular endothelial growth factor-C （VEGF-C） and its receptor （VEGFR-2 2,3,3） in patients＇ serum with acute leukemia.Methods We detected the percentage of CECs in 66 cases of acute leukemua patients using flow cytometry （FCM） and the level of VEGF-C and VEGFR-2 in serum from 66 acute leukemua patients by ELISA.And dynamic observation of 43 patients with AL changes before and after treatment.Results 1.The comparative counting of CECs in the peripheral blood of AL patients at the time of diagnosis as well as CR group and NR group were significantly higher than control group （P ＜0.05） The comparative counting of CECs of in the peripheral blood of CR group were decreased than NR group （P＜0.05）.2.The levels of VEGF-C and VEGFR-2 of AL patients at the time of diagnosis were significantly higher than control group （P＜0.05）,there was a significant difference between the two groups （P＜0.01）.Patients＇ serum levels of VEGF-C and VEGFR-2 were reduced after treatment,and there was a significant difference between the two groups （P＜0.01）.Conclusions In patients with acute leukemia,comparative counting of the CECs is increased significantly in the peripheral blood.Comparative counting of CR group is decreased,as compared with that of NR group after treatment.The levels of VEGF-C,VEGFR-2 related to the growth regulation of CECs are significantly higher than those of control group.There is a certain relationship between progression of disease,curative effect and prognosis and the levels of CECs,VEGF-C and VEGFR-2 in patients with acute leukemia.