CSF-1及其受体与早期自然流产关系的研究

The Relationship of CSF-1 and Its Receptor with Early Spontaneous Abortion

目的探讨母胎界面局部细胞因子CSF-1及其受体与早期自然流产的发生的关系.方法采集2009年8月至2011年12月间来云南省第三人民医院进行人工流产及自然流产病例的绒毛及脱膜组织各30例,采用放射免疫法测定2组外周血中绒毛膜促性腺激素(HCG)含量,利用免疫组织化学和Western blot检测噬细胞集落刺激因子(CSF-1)及其受体(CSF-1R)表达情况.结果自然流产组HCG值与人工流产组相比明显下降(P<0.05).绒毛及脱膜组织中CSF-1和CSF-1R 2组均有表达,与人工流产组相比,自然流产组均增加(P<0.05).结论适量的CSF-1和CSF-1R浓度维持妊娠的继续,浓度过高则不利于妊娠,可能是自然流产的原因之一.

Objective To investigate the relationship of Maternal-fetal interface local cytokine CSF-1 and its receptors with the occurrence of the early spontaneous abortion.Methods From August 2009 to December 2011,we collected 30 villi tissue samples with artificial abortion and 30 villi tissue samples with spontaneous abortion.At the same time,we collected 30 villi tissue samples with artificial abortion and 30 decidua tissue samples with spontaneous abortion,and 30 decidua tissue samples with spontaneous abortion.The human chorionic gonadotropin （HCG） was detected by radioimmunoassay in each group.The expressions of colony Stimulating Factor 1 （CSF-1） and colony Stimulating Factor 1 receptor （CSF-1R） were detected by Immunohistochemical staining and Western Blot in the villi tissue and decidua tissue in every group.Results The levels of HCG in the spontaneous abortion group were lower than those in the artificial abortion group （P＜0.05）.The protein expression of CSF-1 and CSF-1R about the artificial abortion and spontaneous abortion in the the spontaneous abortion group were found.The protein expression levels of CSF-1 and CSF-1R about the artificial abortion and spontaneous abortion in the the spontaneous abortion group were higher than the artificial abortion group by immunohistochemical staining and Western Blot （P＜0.05）.Conclusions The right concentrations of CSF-1 and CSF-1R maintain the pregnancy continued.However,The higher concentrations of CSF-1 and CSF-1R may be one of the factors leading to spontaneous abortion.