摘要
以人工控制劣变处理(温度45℃,相对湿度100%)3 d的玉米杂交种‘郑单958’种子为材料,通过cDNA-AFLP技术,共获得约5 400个转录本。对差异片段进行测序分析,最终获得122个种子劣变相关基因序列(DRS),其中,上调表达74个,下调表达48个,功能涉及初生代谢、次生代谢、蛋白代谢、转录与调控、胁迫响应、信号转导和运输。采用qRT-PCR技术分析了11个上调表达差异基因的表达模式,结果显示不同基因的表达模式存在较大差异,说明不同基因在种子劣变中可能扮演不同角色。
Hybrid seeds of maize （Zea mays） cultivar ＇Zhengdan 958＇ after 3 d of controlled deterioration treatment （CDT） （temperature： 45℃, relative humidity： 100%） were used as the materials and about 5 400 transcript-derived fragments （TDFs） were obtained by cDNA-AFLP approach. 122 deterioration relevant sequences （DRSs） obtained by bioinformatics analysis, in which 74 were up-regulated and 48 were down-regulated, were involved in primary metabolism, secondary metabolism, protein metabolism, transcription and regulation, stress response, signal pathway and transportation. The expression patterns of 11 differential genes up-regulated were analysed by qRT-PCR and the result showed that these genes displayed different expression patterns, suggesting that they might have different roles in seed deterioration.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第7期973-982,共10页
Plant Physiology Journal
基金
国家自然科学基金项目(31371709)
