拟南芥AtrbohD和AtrbohF缺失对叶蛋白质组的影响

Effects of Defi ciency in AtrbohD and AtrbohF on Changes in Leaf Proteome in Arabidopsis thaliana

拟南芥NADPH氧化酶AtrbohD和AtrbohF在脱落酸(abscisic acid,ABA)抑制主根伸长、ABA诱导气孔关闭以及植物应答干旱、盐及病菌侵染等逆境胁迫反应中发挥重要作用,但这2个蛋白亚基缺失对拟南芥(Arabidopsis thaliana)蛋白质组的影响还未见报道。我们以营养土中生长16 d的野生型及AtrbohD和AtrbohF双基因突变体atrbohD1/F1叶片为材料进行蛋白组学分析,在双向电泳图谱上可分辨出约1 000个蛋白点,且蛋白表达谱存在差异。选取42个显著差异蛋白点进行MALDI-TOF/TOF质谱鉴定,成功鉴定出20个差异蛋白,这些蛋白主要与氧化还原、能量代谢、蛋白代谢、转录和信号传导等相关,还有一些蛋白功能未知。

NADPH oxidase AtrbohD and AtrbohF play essential roles in abscisic acid （ABA）-inhibited primary root growth, ABA-induced stomatal closure, and in response to adverse stress conditions including disease infection, drought and salt stress in Arabidopsis. However, effects of deficiency in the two protein subunits on change in leaf proteome have not been reported. Here, leaf proteomic analysis of wild type （WT） and double mutant atrbohD1/F1 of AtrbohD and AtrbohF after grown in soil for 16 days were performed. About 1 000 protein spots in WT and the mutant were detected in the two-dimensional electrophoresis gels, and clear difference in protein profiles was observed. The 42-protein spots were selected based on their differential expression be- tween WT and the mutant, and 20 proteins of which were identified by MALDI-TOF/TOF mass spectrometry. Most of proteins identified are involved in multiple cellular processes including redox, energy metabolism, protein metabolism, transcription and signal transduction. Some other identified proteins are functional unknown.