中国食蟹猴脑磁共振图像模板的构建

Construction of MRI template of the chinese cynomolgus monkeys brain

目的建立中国食蟹猴脑结构T1图像模板,分析其脑灰质、白质及脑脊液组织概率分布图。方法 16只健康中国食蟹猴3.0TMRI脑的高分辨3D矢状位T1WI采集,使用模糊C均值聚类(fuzzy c-meass clusteriag Algorithm,FCM)法对每例图像进行组织分割、插值、空间标准化及图像平滑,建立脑白质、灰质、脑脊液组织概率图和个体脑模板。对所得图像进行配准及平均后,再经过VBM流程分析测试获得食蟹猴MRI图像模板。结果建立了正常中国食蟹猴脑结构3D模板,并获得了食蟹猴脑白质、灰质、脑脊液三种组织的概率图,清晰地显示了全脑T1加权对比结构。结论建立MRI图像模板和组织概率图为中国食蟹猴脑结构VBM分析和fMRI定位提供了重要的基础和便利。

Objective To establish a T1 image template of the Chinese cynomolgus monkeys（Macaca fascicularis）brain and to perform a analysis brain tissue by developing a digital multi-atlas of grey matter,whiter matter and cerebrospinal fluid.Methods Sixteen healthy Chinese cynomolgus monkeys were scanned by high resolution 3.0TMagnatic Resonance Imaging（MRI）to acquire sagittal 3DT1 WI images.An individual brain template and tissue digital atlases of grey matter was conducted,white matter and cerebrospinal fluid;segmentation,interpolation,spatial normalization and image smoothing of the MRIs were carried out using the Fuzzy c-means Clustering Algorithm（FCM）.When co-registration and an average on the reference images were performed,cynomolgus monkeys brain atlases was then constructed after voxel-based morphometry（VBM）process analysis and testing of the multi-atlas.Results The 3D MRI template of healthy Chinese cynomolgus monkeys was established three tissue digital atlases of grey matter,white matter and cerebrospinal fluid were developed,distinctly indicating the whole brain T1-weighted contrast structure.Conclusion The construction of MRI template of the cynomolgus monkeys brain provides a foundation and convenience for brain functional orientation with fMRI and brain structure analysis with VBM.