人增生性瘢痕表皮干细胞修复裸鼠皮肤创面的实验研究

Experimental study of skin wound healing with epidermal stem cells from human hypertrophic scar in nude mice

目的 探讨人成熟期增生性瘢痕来源的表皮干细胞（ESCs）修复裸鼠皮肤缺损的效果.方法 选择40只裸鼠,在每只裸鼠背部制造一直径1 cm的创面,随机分为实验组和对照组,每组20只.实验组在创面上给予人增生性瘢痕-ESCs＋红霉素眼膏治疗,对照组创面只外用红霉素跟膏,其他处理一致.观察裸鼠背部创面愈合时间及14 d时创面组织的修复情况、Ⅰ和Ⅲ型胶原、表皮生长因子（EGF）、成纤维生长因子（FGF2）、转化生长因子（TGFβ1和TGFβ2）的表达情况.结果 创面愈合时间实验组为（20.8±0.84）d,对照组为（25.6±0.89） d;HE染色显示实验组的血管化数目为（11.60±0.55）支,对照组为（8.04±0.33）支;免疫组织化学检测显示实验组皮肤的Ⅰ、Ⅲ型胶原及EGF、FGF2、TGFβ1和TGFβ2分泌能力更强;这些指标在2组之间的差异均具有统计学意义（P＜0.05）.结论 增生性瘢痕-ESCs能够促进创面的愈合,其机制可能是促进了创面组织血管化和生长因子的分泌.

Objective To investigate the effect of epidermal stem cells from human hypertrophic scar （HS-ESCs）on the skin wound healing in nude mice.Methods 40 mice were randomly divided into two groups as experimental group（n =20） and control group（n =20）.Wounds,1 cm in diameters,were made on every mouse back.The wounds were treated with HS-ESCs and erythromycin ointment in experimental group,or only with erythromycin ointment in control group.The wound healing was observed during the following 14 days.The expression of collagen-Ⅰ,collagen-Ⅲ,epidermal growth factor （EGF）,fibroblast growth factor （FGF2）,transforming growth factor （TGFβ1 and TGFβ2） were studied.Results The wound healing time in the experimental group was （20.8 ± 0.84） d,which was （25.6 ± 0.89） d in the control group.HE staining revealed that the extent of vascularization in the experimental group was 11.60 ± 0.55,while it was 8.04 ± 0.33 in the control group.Immunochemistry analysis showed the expression of collagen-Ⅰ,collagen-Ⅲ,EGF,FGF2,TGFβ1 and TGFβ2 in the experimental group were significantly higher,compared with those in control group （P ＜ 0.05）.Conclusion HS-ESCs may promote wound healing through enhancement of the vascularization of the wound tissue and the expression of growth factors.