乙型肝炎X蛋白与乙型肝炎病毒标志物定量及HBVDNA检测的关系研究

Relationship between HBV X Protein with HBV DNA and Hepatitis B Markers in Patients with Viral Hepatitis B

目的检测慢性乙型肝炎(CHB)患者血清X蛋白含量、HBVDNA、前S1抗原(Pre-S1)、乙型肝炎病毒标志物(HB-VM),探讨他们之间的关系,评估其在HBV感染中的临床价值。方法采用酶联免疫吸附试验(ELISA)检测乙型肝炎患者血清中X蛋白,PreS1;时间分辨法定量测定HBVM,FQ-PCR定量检测HBVDNA。乙型肝炎X蛋白对疾病的影响。结果肝炎组患者的X蛋白阳性率高于健康对照组,肝癌组X蛋白阳性率高于肝炎组,乙型肝炎DNA测定值阳性X蛋白阳性率高于阴性,HBeAg抗原阳性中X蛋白的检出率也高于阴性,并且差异均有统计学意义。结论乙型肝炎X蛋白在疾病监测中有一定的临床意义,并且对病毒的复制有影响。

Objective To detect the clinical significance of HBV X protein in patients with viral hepatitis B. Detection of HBVDNA, former S1 antigen （Pre-S1）, hepatitis B virus markers （HB-VM） of patients, to explore the relationship between them, assess their clinical HBV infection in value. Methods By enzyme-linked immunosorbent assay （ELISA） to detect serum hepatitis BXprotein, PreS1;Time-resolved quantitative determination HBVM, FQ-PCR quantitative detection HBVDNA. HBV X protein on the disease. Results X protein of hepatitis patients was higher in the healthy control group, the group X protein positive rate of liver cancer in hepatitis A, hepatitis B DNA positive X protein was measured value is higher than the negative, HBeAg-positive antigen detection rate in the X protein also higher than the negative, and the differences were statistically significant. Conclusions HBV X protein has clinical significance in disease surveillance, and the replication of the virus affected.