低功率激光修复末端病大鼠跟腱早期愈合中转化生长因子β1的表达

Transforming growth factor-beta 1 expression in early enthesiopathy of Achilles tendon in rats after low-level laser therapy

背景:低功率激光是一种治疗性激光,其在运动医学领域内应用价值潜力很大。稿目的:观察低功率激光照射后,末端病大鼠跟腱转化生长因子β1表达的变化,探讨低功率激光修复末端病大鼠跟腱的机制。方法:4周龄雄性Wistar大鼠96只,随机分为空白对照组(n=8)、造模组(n=88),造模组又分为造模对照组(n=48)和不同时间的激光照射组:激光照射1,2,3,7,14 d组(n=8)。造模组大鼠进行5周的电刺激跳跃之后,对激光照射组的大鼠跟腱按要求进行激光照射。用实时定量PCR方法测定跟腱内转化生长因子β1 mRNA的表达;双抗体夹心ABC-ELISA法测试转化生长因子β1的蛋白含量。结果与结论:造模即刻,与空白对照组比较,造模对照组大鼠跟腱转化生长因子β1明显升高(P<0.05)。随着时间的推移,转化生长因子β1逐渐下降,与激光照射组比较,造模对照组大鼠跟腱转化生长因子β1在第7,14天时明显下降(P<0.05)。低功率激光对末端病大鼠跟腱早期愈合有一定的促进作用:可延缓末端病大鼠跟腱转化生长因子β1的浓度下降,使转化生长因子β1在7-14 d内保持较高水平,对细胞外基质起着重要的调节作用。

BACKGROUND： Low-level laser is a kind of therapeutic laser and has groat potential application value in the field of sports medicine. OBJECTIVE： To discuss the repairing mechanism of low-level laser therapy in early enthesiopathy of achilles tendon in rats through observing the change of transforming growth factor-β1 expression after low-level laser therapy. METHODS： A total of 96 male Wistar rats aged 4 weeks were randomly divided into control group （n=8） and model group （n=88）. Then model group was assigned randomly into two subgroups： model control group （n=48） and laser groups （n=40）. The laser group was further allocated to 1,2, 3, 7, 14 days of laser radiation, with eight rats in each. The rats of model group were trained by electrical stimulation for 5 weeks, and the rats of laser groups received laser radiation. The expression of transforming growth faotor-β1 mRNA level in Achilles tendon was analyzed by real-time quantitative PCR. The transforming growth factor-β1 content was tested by doubleantibody ABC-ELISA. RESULTS AND CONCLUSION： The transforming growth factor-~l in model control group was increased significantly compared with control group immediately after modeling （P 〈 0.05）. Then, transforming growth factor-131 was gradually decreasing as the time prolonged. Compared with laser groups, transforming growth factor-βl in model control group was decreased significantly at 7 and 14 days （P 〈 0.05）. Low-level laser therapy promotes the early healing of achilles tendon enthesiopathy in rats, delays the decrease of transforming growth factor-βl content, and maintains high level of transforming growth factor-β1 at 7-14 days. Low-level laser therapy plays an important role in regulating extracellular matrix.