蛋白质组学相关技术在骨关节炎研究中的应用与进展

Progress of proteomics technology and its application in osteoarthritis

背景:蛋白质组学研究已经彻底改革了研究疾病的方式,提供了解开骨关节炎病理生理机制的途径。目的:了解蛋白质组相关技术的研究成果及其在骨关节炎早期诊断、治疗效应和发病机制的分子标志物研究取得的进展。方法:通过中国期刊全文数据库、中国生物医学文献数据库、重庆维普中文科技期刊数据库和万方数据知识服务平台,手工和在线检索已发表的关于蛋白质组学技术和骨关节炎相关的研究。英文文献采用以下数据库检索:MEDLINE数据库,Elsevier:ScienceDirect数据库,BIOSIS Previews数据库。纳入文献为采用严格相关和信息可靠的研究。结果与结论:蛋白质组学技术的成果为骨关节炎早期诊断、治疗效应和发病机制的分子标志物研究取得了一定的进展。研究证实了蛋白质组学在筛选骨关节炎分子标志物的研究中有较大的应用价值,其相对于单一蛋白鉴定具有通量高、速度快的特点,对骨关节炎的诊断和药物靶点鉴定发挥了重要作用。蛋白质组数据库是蛋白质组研究水平的标志和基础。生物信息学的发展已给蛋白质组研究提供了更方便有效的计算机分析软件。

BACKGROUND： Proteomics research has thoroughly reformed the way to study the diseases, and provides the understanding of the mechanism of osteoarthritis pathological physiology. OBJECTIVE： To focus on the research progress of proteornics technology in osteoarthritis early diagnosis, therapeutic effect and pathogenesis molecular markers. METHODS： Researches on proteomics technology and osteoarthritis published in journals were identified manually and on-line retrieved by using SinoMed, Chongqing VIP databasel Wanfang Data and CNKI database. Those researches reported in English journals were identified using MEDLINE, Elsevier： ScienceDirect, and BIOSIS Previews database, Selected studies should describe a related and reliable study defined by strict screening and diagnostic criteria. RESULTS AND CONCLUSION： Preteomics technology for the osteoarthritis in early diagnosis, treatment effect and pathogenesis molecular markers has achieved a certain progress. Research showed that proteomics has great application value in screening osteoarthritis molecular markers, and has the characteristics of high flux and high speed compared with single protein identification, so it plays an important role in the diagnosis of osteoarthritis and drug target identification. Proteome database is a sign of prcteome research level. The development of the bioinformatics has provided a more convenient and effective computer analysis Software for proteomic study.