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D-乳酸合成阻断对肺炎克雷伯氏菌的1,3-丙二醇发酵的影响 被引量：1

Influence of blocking formation of D-lactic acid on 1,3-propanediol fermentation by Klebsiella pneumoniae
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摘要 利用Red同源重组技术,快速敲除肺炎克雷伯氏菌中的编码D-乳酸脱氢酶的两个基因——ldhA和dld,获得KG1-1和KG1-2两个突变株,并研究了敲除编码D-乳酸脱氢酶基因丧失合成D-乳酸的KG1-1菌株的1,3-PD产量和菌体生长变化,实验结果表明乳酸合成缺失对现有工艺1,3-丙二醇发酵无影响。 The impact of blocking D-lactate formation by direct knocking out the genes of D-lactic dehydrogenase in Klebsiella pneumoniae was investigated. The two genes of ldhA and dld were knocked out separately to construct two mutants of KG1-1 and KG1-2. The results showed that KGI-1 was a D-lactate deficient mutant, while its 1,3-PD production and cell growth were not influenced on the present technology.
作者 周佳佳 高黎荣 刘龙飞 张书翠 付水林 宫衡
机构地区 华东理工大学生物反应器工程国家重点实验室
出处 《工业微生物》 CAS CSCD 2014年第4期24-27,共4页 Industrial Microbiology
关键词 肺炎克雷伯氏菌 D-乳酸脱氢酶 1 3-丙二醇 RED同源重组 Klebsiella pneumoniae D-lactate dehydrogenase 1,3-propanediol Red recombination
分类号 TQ923 [轻工技术与工程—发酵工程]
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参考文献8

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共引文献4

同被引文献14

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  • 10张宏蕊,金平,刘树臣.发酵产1,3-丙二醇的关键酶研究进展[J].化学与生物工程,2011,28(3):1-4. 被引量：3

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工业微生物
2014年 第4期

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